Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk
| Autor: | Joseph Hershenhorn, Shmuel Galili, Xiaonan Xie, Evgenia Dor, Aharon Bellalou, Evgeny Smirnov, Koichi Yoneyama, Orit Amir-Segev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ethyl methanesulfonate
Mutant Population Strigolactone Plant Science Orobanche crenata chemistry.chemical_compound chickpea chlorophyll Cultivar strigolactone education chickpea phenotype Ecology Evolution Behavior and Systematics education.field_of_study Ecology biology Botany Wild type food and beverages broomrape resistance biology.organism_classification carotenoid Horticulture chemistry Germination QK1-989 anthocyanin |
| Zdroj: | Plants; Volume 10; Issue 12; Pages: 2552 Plants, Vol 10, Iss 2552, p 2552 (2021) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants10122552 |
| Popis: | Chickpea (Cicer arietinum L.) is a major pulse crop in Israel grown on about 3000 ha spread, from the Upper Galilee in the north to the North-Negev desert in the south. In the last few years, there has been a gradual increase in broomrape infestation in chickpea fields in all regions of Israel. Resistant chickpea cultivars would be simple and effective solution to control broomrape. Thus, to develop resistant cultivars we screened an ethyl methanesulfonate (EMS) mutant population of F01 variety (Kabuli type) for broomrape resistance. One of the mutant lines (CCD7M14) was found to be highly resistant to both Phelipanche aegyptiaca and Orobanche crenata. The resistance mechanism is based on the inability of the mutant to produce strigolactones (SLs)—stimulants of broomrape seed germination. LC/MS/MS analysis revealed the SLs orobanchol, orobanchyl acetate, and didehydroorobanchol in root exudates of the wild type, but no SLs could be detected in the root exudates of CCD7M14. Sequence analyses revealed a point mutation (G-to-A transition at nucleotide position 210) in the Carotenoid Cleavage Dioxygenase 7 (CCD7) gene that is responsible for the production of key enzymes in the biosynthesis of SLs. This nonsense mutation resulted in a CCD7 stop codon at position 70 of the protein. The influences of the CCD7M14 mutation on chickpea phenotype and chlorophyll, carotenoid, and anthocyanin content were characterized. |
| Databáze: | OpenAIRE |
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