Gulp1 controls Eph/ephrin trogocytosis and is important for cell rearrangements during development
| Autor: | Rüdiger Klein, Jana Lindner, Yunyun Huang, Rudolf Winklbauer, Olivia Luu, Louise Gaitanos, Jingyi Gong, Thomas N Gaitanos |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Trogocytosis Endocytic cycle Erythropoietin-producing hepatocellular (Eph) receptor Adaptor Signaling Protein Signal transducing adaptor protein Cell Biology Biology Cell biology 03 medical and health sciences 0302 clinical medicine Ephrin 030217 neurology & neurosurgery Trans-endocytosis 030304 developmental biology Dynamin |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201901032 |
| Popis: | Trogocytosis, in which cells nibble away parts of neighboring cells, is an intercellular cannibalism process conserved from protozoa to mammals. Its underlying molecular mechanisms are not well understood and are likely distinct from phagocytosis, a process that clears entire cells. Bi-directional contact repulsion induced by Eph/ephrin signaling involves transfer of membrane patches and full-length Eph/ephrin protein complexes between opposing cells, resembling trogocytosis. Here, we show that the phagocytic adaptor protein Gulp1 regulates EphB/ephrinB trogocytosis to achieve efficient cell rearrangements of cultured cells and during embryonic development. Gulp1 mediates trogocytosis bi-directionally by dynamic engagement with EphB/ephrinB protein clusters in cooperation with the Rac-specific guanine nucleotide exchange factor Tiam2. Ultimately, Gulp1’s presence at the Eph/ephrin cluster is a prerequisite for recruiting the endocytic GTPase dynamin. These results suggest that EphB/ephrinB trogocytosis, unlike other trogocytosis events, uses a phagocytosis-like mechanism to achieve efficient membrane scission and engulfment. |
| Databáze: | OpenAIRE |
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