Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2
| Autor: | Chunyan Zhang, Chengrong Lu, Yaping Tian, Xinyu Wen, Yuan Luo, Jing Gao, Ling Wang, Lianning Duan, Zi Wang, Pengjun Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aging Cell cycle checkpoint Carcinoma Hepatocellular Time Factors Article Subject Cell Survival Antineoplastic Agents Apoptosis Biology Biochemistry 03 medical and health sciences microRNA Humans Computer Simulation Gene Regulatory Networks Viability assay Protein Interaction Maps lcsh:QH573-671 Cell Proliferation Regulation of gene expression Dose-Response Relationship Drug lcsh:Cytology Cell growth Gene Expression Profiling Liver Neoplasms Computational Biology Cell Biology General Medicine Cell Cycle Checkpoints Hep G2 Cells Hydrogen Peroxide Cell cycle Cell biology Gene expression profiling Gene Expression Regulation Neoplastic MicroRNAs Oxidative Stress 030104 developmental biology Reactive Oxygen Species Algorithms Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2016 (2016) |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Although both oxidative stress and microRNAs (miRNAs) play vital roles in physiological and pathological processes, little is known about the interactions between them. In this study, we first described the regulation of H2O2in cell viability, proliferation, cycle, and apoptosis of human hepatocellular carcinoma cell line HepG2. Then, miRNAs expression was profiled after H2O2treatment. The results showed that high concentration of H2O2(600 μM) could decrease cell viability, inhibit cell proliferation, induce cell cycle arrest, and finally promote cell apoptosis. Conversely, no significant effects could be found under treatment with low concentration (30 μM). miRNAs array analysis identified 131 differentially expressed miRNAs (125 were upregulated and 6 were downregulated) and predicted 13504 putative target genes of the deregulated miRNAs. Gene ontology (GO) analysis revealed that the putative target genes were associated with H2O2-induced cell growth arrest and apoptosis. The subsequent bioinformatics analysis indicated that H2O2-response pathways, including MAPK signaling pathway, apoptosis, and pathways in cancer and cell cycle, were significantly affected. Overall, these results provided comprehensive information on the biological function of H2O2treatment in HepG2 cells. The identification of miRNAs and their putative targets may offer new diagnostic and therapeutic strategies for liver cancer. |
| Databáze: | OpenAIRE |
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