Increased Circulatory Extrarenal 1α,25-Dihydroxyvitamin D3 in Bilaterally Nephrectomized Rats
| Autor: | Hakan Bulut, Tuncay Kuloglu, Ersoy Baydar, Özgür Özöner, Durrin Ozlem Dabak, Murat Dabak |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Lipopolysaccharide Endocrinology Diabetes and Metabolism medicine.medical_treatment Inflammation Kidney Nephrectomy chemistry.chemical_compound Immune system In vivo Internal medicine medicine Immunology and Allergy Animals Rats Wistar Vitamin D business.industry Rats Endocrinology chemistry Calcitonin Circulatory system medicine.symptom business Biomarkers Bilateral Nephrectomy |
| Zdroj: | Endocrine, metabolicimmune disorders drug targets. 20(9) |
| ISSN: | 2212-3873 |
| Popis: | Background: Extrarenal 1α,25-dihydroxyvitamin D3 (1,25-D) locally produced by immune cells plays crucial roles in the regulation of the immune system. However, in vivo status of extrarenal 1,25-D and 25-hydroxyvitamin D (25-D) in acute inflammatory conditions are unknown. Objective: The aim of this study was to determine the extrarenal 1,25-D level in circulation in bilaterally nephrectomized rats, induced by low-dose lipopolysaccharide (LPS). Methods: Renal 1,25-D synthesis was terminated through bilateral nephrectomy in rats. The rats received intraperitoneal LPS (50 μg/kg BW) three times and the experiment was ended 24 hours after nephrectomy. Serum 1,25-D, 25-D, calcium, phosphorus, intact parathyroid hormone, and calcitonin levels were measured and immunohistochemistry was applied to detect the sources of extrarenal 1,25- D synthesis. Results: Circulatory 1,25-D concentration remarkably increased in both LPS-treated and non-treated bilaterally nephrectomized rats. Elevated circulatory 1,25-D did not have hypercalcemic endocrinal effects. The increased 1,25-D level also resulted in a concurrent rapid and dramatic depletion of circulatory 25-D. Conclusions: Extrarenal 1,25-D could enter into the systemic circulation and, therefore, might have systemic effects besides its autocrine and paracrine functions. |
| Databáze: | OpenAIRE |
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