NKT Cells Provide Help for Dendritic Cell-Dependent Priming of MHC Class I-Restricted CD8+ T Cells In Vivo
| Autor: | Reinhold Schirmbeck, Detlef Stober, Jörg Reimann, Ieva Jomantaitė |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytotoxicity Immunologic Male Adoptive cell transfer T cell Lymphocyte Cooperation Molecular Sequence Data Immunology Priming (immunology) Bone Marrow Cells chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Ceramides Lymphocyte Activation Antigens CD1 Mice T-Lymphocyte Subsets MHC class I Tumor Cells Cultured medicine Animals Immunology and Allergy Cytotoxic T cell Amino Acid Sequence Cells Cultured Bone Marrow Transplantation Mice Knockout biology Chemistry H-2 Antigens Histocompatibility Antigens Class II hemic and immune systems Dendritic Cells Dendritic cell Natural killer T cell Adoptive Transfer Killer Cells Natural Mice Inbred C57BL CTL medicine.anatomical_structure biology.protein Female Immunization Antigens CD1d T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Immunology. 170:2540-2548 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.170.5.2540 |
| Popis: | Dendritic cells (DC) are potent APCs for naive T cells in vivo. This is evident by inducing T cell responses through adoptive DC transfer. Priming specific CTL responses in vivo often requires “help”. We study alternative sources of help in DC-dependent priming of MHC class I-restricted CTL. Priming an anti-viral CTL response in naive B6 mice by adoptive transfer of antigenic peptide-pulsed DC required CD4+ T cell help. CTL priming was facilitated by providing MHC class II-dependent specific help. Furthermore, transfers of MHC class II-deficient pulsed DC into naive, normal hosts, or DC transfers into naive, CD4+ T cell-depleted hosts primed CTL inefficiently. Pretreatment of DC with immune-stimulating oligodeoxynucleotides rendered them more efficient for CD4+ T cell-independent priming of CTL. DC copresenting a Kb-binding antigenic peptide and the CD1d-binding glycolipid α-galactosyl-ceramide efficiently primed CTL in a class II-independent way. To obtain NKT cell-dependent help in CTL priming, the same DC had to present both the peptide and the glycolipid. CTL priming by adoptive DC transfer was largely NK cell-dependent. The requirement for NK cells was only partially overcome by recruiting NKT cell help into DC-dependent CTL priming. NKT cells thus are potent helper cells for DC-dependent CTL priming. |
| Databáze: | OpenAIRE |
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