A Novel Alkaloid from Marine-Derived Actinomycete Streptomyces xinghaiensis with Broad-Spectrum Antibacterial and Cytotoxic Activities

Autor: Wen-Ce Jiao, Xin-Qing Zhao, Fenghua Zhang, Joo-Won Suh, Jiehan Hu
Rok vydání: 2013
Předmět:
Aquatic Organisms
Light
lcsh:Medicine
Bacillus subtilis
medicine.disease_cause
Pathology and Laboratory Medicine
Spectrum analysis techniques
Diagnostic Radiology
Medicine and Health Sciences
lcsh:Science
Fungal Pathogens
Multidisciplinary
biology
Antimicrobials
Radiology and Imaging
Physics
Electromagnetic Radiation
Streptomyces xinghaiensis
Drugs
Magnetic Resonance Imaging
Streptomyces
Acinetobacter baumannii
Anti-Bacterial Agents
Actinobacteria
Chemistry
Medical Microbiology
Physical Sciences
Pathogens
Research Article
Imaging Techniques
Antineoplastic Agents
Microbial Sensitivity Tests
Mycology
Image Analysis
Research and Analysis Methods
Microbiology
Inhibitory Concentration 50
Alkaloids
NMR spectroscopy
Diagnostic Medicine
Cell Line
Tumor
Microbial Control
medicine
Humans
Escherichia coli
Microbial Pathogens
Pharmacology
Bacteria
Pseudomonas aeruginosa
lcsh:R
Chemical Compounds
Organisms
Biology and Life Sciences
biology.organism_classification
Retraction
Fermentation
Antibacterials
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 10, p e75994 (2013)
ISSN: 1932-6203
Popis: Due to the increasing emergence of drug-resistant bacteria and tumor cell lines, novel antibiotics with antibacterial and cytotoxic activities are urgently needed. Marine actinobacteria are rich sources of novel antibiotics, and here we report the discovery of a novel alkaloid, xinghaiamine A, from a marine-derived actinomycete Streptomyces xinghaiensis NRRL B24674(T). Xinghaiamine A was purified from the fermentation broth, and its structure was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR spectrum as well as mass spectrometry. Xinghaiamine A was identified to be a novel alkaloid with highly symmetric structure on the basis of sulfoxide functional group, and sulfoxide containing compound has so far never been reported in microorganisms. Biological assays revealed that xinghaiamine A exhibited broad-spectrum antibacterial activities to both Gram-negative persistent hospital pathogens (e.g. Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli) and Gram-positive ones, which include Staphylococcus aureus and Bacillus subtilis. In addition, xinghaiamine A also exhibited potent cytotoxic activity to human cancer cell lines of MCF-7 and U-937 with the IC50 of 0.6 and 0.5 µM, respectively.
Databáze: OpenAIRE
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