Revealing mitogenome-wide DNA methylation and RNA editing of three Ascomycotina fungi using SMRT sequencing
| Autor: | Chaoxia Wang, Zhang Yaozhou, Li Liu, Zhang Shujun, Wu Yuqian, Du Simiao, Chen Yujiao, Li Dongmei, Feng Jianhua |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Acclimatization Ophiocordyceps sinensis Bassiana Deep sequencing 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cordyceps militaris Beauveria Medicine Chinese Traditional Molecular Biology Genetics biology Gene Expression Profiling Genetic Variation Cell Biology DNA Methylation biology.organism_classification 030104 developmental biology chemistry RNA editing DNA methylation Cordyceps Genome Mitochondrial Molecular Medicine RNA Editing Transcriptome 030217 neurology & neurosurgery DNA Single molecule real time sequencing |
| Zdroj: | Mitochondrion. 51 |
| ISSN: | 1872-8278 |
| Popis: | Beauveria bassiana, Cordyceps militaris and Ophiocordyceps sinensis (Ascomycotina) are traditional Chinese medicines. Here, mitogenomes of these three Ascomycotina fungi were sequenced and de-novo assembled using single-molecule real-time sequencing. The results showed that their complete mitogenomes were 31,258, 31,854 and 157,584 bp, respectively, with sequencing depth approximately 278,760×, 326,283× and 69,385×. Types of repeat sequences were mainly (AA)n, (AAT)n, (TA)n and (TATT)n. DNA methylation motifs were revealed in DNA modifications of these three fungi. We discovered new models of RNA editing through analysis of transcriptomes from B. bassiana and C. militaris. These data lay a solid foundation for further genetic and biological studies about these three fungi, especially for elucidating the mitogenome evolution and exploring the regulatory mechanism of adapting environment. |
| Databáze: | OpenAIRE |
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