Peptides inhibiting heparanase procoagulant activity significantly reduce tumour growth and vascularisation in a mouse model
| Autor: | Benjamin Brenner, Inna Kogan, Yona Nadir, Elena Axelman, Mifleh Tatour, Yonatan Crispel, Neta Nevo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Angiogenesis Melanoma Experimental Inflammation 030204 cardiovascular system & hematology Biology Thromboplastin Sepsis 03 medical and health sciences Tissue factor Mice 0302 clinical medicine Internal medicine Cell Line Tumor medicine Animals Humans Heparanase Glucuronidase Neovascularization Pathologic Cancer Hematology medicine.disease 030104 developmental biology Endocrinology Coagulation Cell culture Cancer research medicine.symptom Neoplasm Recurrence Local Peptides |
| Zdroj: | Thrombosis and haemostasis. 116(4) |
| ISSN: | 2567-689X |
| Popis: | SummaryHeparanase is implicated in angiogenesis and tumour progression. We previously demonstrated that heparanase might also affect the haemostatic system in a non-enzymatic manner. It forms a complex and enhances the activity of the blood coagulation initiator tissue factor (TF). Peptides that we generated from TF pathway inhibitor (TFPI)-2, which inhibit heparanase procoagulant activity, were recently demonstrated to attenuate inflammation in a sepsis mouse model. The present study was designated to explore peptides effects on tumour growth and vascularisation. Cell lines of mouse melanoma (B16), mouse breast cancer (EMT-6), and human breast cancer (MDA-231) were injected subcutaneously to mice. Inhibitory peptides 5, 6 and 7 were injected subcutaneously in the area opposite to the tumour side. In the three tumour cell lines, peptides 5, 6 and 7 inhibited tumour growth and vascularisation in a dose-dependent manner, reaching a 2/3 reduction compared to control tumours (p |
| Databáze: | OpenAIRE |
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