Examining the Role of the Surfactant Family Member SFTA3 in Interneuron Specification

Autor: Friedrich Paulsen, Sandy Becker, Lars Bräuer, Laura Grabel, Nickesha C. Anderson, Christopher Y. Chen, Martin Schicht, Christopher E Stoddard
Rok vydání: 2018
Předmět:
0301 basic medicine
Surfactants
Cellular differentiation
lcsh:Medicine
Gene Expression
Biochemistry
Transcriptome
Animal Cells
Medizinische Fakultät
Gene expression
Clustered Regularly Interspaced Short Palindromic Repeats
lcsh:Science
Materials
Neurons
Multidisciplinary
Brain
Cell Differentiation
Genomics
Cell biology
medicine.anatomical_structure
Physical Sciences
embryonic structures
Cytochemistry
Cellular Types
Transcriptome Analysis
Immunocytochemistry
Neuronal Differentiation
Research Article
Pulmonary Surfactant-Associated Proteins
Ganglionic eminence
Interneuron
Neurogenesis
Materials Science
Biology
Cell Line
03 medical and health sciences
Interneurons
Genetics
medicine
Humans
Cell Lineage
ddc:610
Progenitor cell
Gene
Transcription factor
Embryonic Stem Cells
Progenitor
lcsh:R
Biology and Life Sciences
Computational Biology
Cell Biology
Genome Analysis
Embryonic stem cell
030104 developmental biology
Cellular Neuroscience
lcsh:Q
Neuroscience
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 11, p e0198703 (2018)
DOI: 10.1101/330621
Popis: The transcription factorNKX2.1, expressed at high levels in the medial ganglionic eminence (MGE), is a master regulator of cortical interneuron progenitor development. To identify gene candidates with expression profiles similar toNKX2.1, previous transcriptome analysis of human embryonic stem cell (hESC)-derived MGE-like progenitors revealedSFTA3as the strongest candidate. Quantitative real-time PCR analysis of hESC-derived NKX2.1-positive progenitors and transcriptome data available from the Allen Institute for Brain Science revealed comparable expression patterns forNKX2.1andSFTA3during interneuron differentiationin vitroand demonstrated highSFTA3expression in the human MGE. AlthoughSFTA3has been well studied in the lung, the possible role of this surfactant protein in the MGE during embryonic development remains unexamined. To determine ifSFTA3plays a role in MGE specification,SFTA3-/- andNKX2.1 -/-hESC lines were generated using custom designed CRISPRs. We show thatNKX2.1KOs have a significantly diminished capacity to differentiate into MGE interneuron subtypes.SFTA3KOs also demonstrated a somewhat reduced ability to differentiate down the MGE-like lineage, although not as severe relative toNKX2.1deficiency. These results suggestNKX2.1andSFTA3are co-regulated genes, and that deletion ofSFTA3does not lead to a major change in the specification of MGE derivatives.
Databáze: OpenAIRE
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