Allelic Variation in the TNF-beta Gene Does Not Explain the Low TNF-beta Response in Patients With Primary Biliary Cirrhosis
| Autor: | Ekkehard D. Albert, Ulrich Spengler, Gert Riethmüller, Elisabeth H. Weiss, Gerald Messer, J. Eisenburg, Gerd R. Pape, G. Honold, Maria C. Jung, Siegfried Scholz |
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| Rok vydání: | 1991 |
| Předmět: |
Autoimmune disease
Liver Cirrhosis Biliary Biliary cirrhosis medicine.medical_treatment Histocompatibility Antigens Class I Immunology Haplotype Histocompatibility Antigens Class II General Medicine Biology medicine.disease Liver disease Cytokine Primary biliary cirrhosis medicine biology.protein Humans Allele Lymphotoxin-alpha Alleles Polymorphism Restriction Fragment Length Phytohaemagglutinin |
| Zdroj: | Scandinavian Journal of Immunology. 34:735-740 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | Autoimmune disorders in humans are often associated with particular alleles of major histocompatibility genes. However, the chronic inflammatory liver disease primary biliary cirrhosis (PBC) has not been found to be correlated with certain haplotypes so far. Interestingly, an impaired production of tumour necrosis factor beta (TNF-beta) upon mitogen stimulation was observed for PBC patients, especially in the immunologically active stages of the disease. Furthermore, the identification of alleles of the TNF-beta gene which differ in one unique amino acid, and in the production of TNF-beta after phytohaemagglutinin stimulation, has prompted the idea of a possible linkage between the impaired TNF-beta response in PBC and the genetic prevalence of a certain TNF haplotype. We report here a rapid method for typing the TNFB*1 and TNFB*2 genes by a standard polymerase chain reaction. PBC patients (n = 60) as well as randomized healthy controls (n = 179) of the Munich area were studied for the occurrence of the TNF alleles. No deviation was found in the PBC collective (0.7) for the TNFB*2 distribution when compared with the control (0.67). |
| Databáze: | OpenAIRE |
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