Application of Gel Permeation HPLC for Lipoprotein Profiling in Dogs
| Autor: | Kenji Iwase, Hisashi Mizutani, Koji Kuriyama, Akihiro Mori, Toshinori Sako, Noriaki Arai, Hisashi Hirose, Itaru Yoshimura |
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| Rok vydání: | 2010 |
| Předmět: |
Very low-density lipoprotein
Chromatography Low-density lipoprotein receptor-related protein 8 General Veterinary Triglyceride Cholesterol Lipoproteins Lipoproteins VLDL High-performance liquid chromatography Lipoproteins LDL chemistry.chemical_compound Dogs High-density lipoprotein chemistry Biochemistry Reference Values Low-density lipoprotein Chromatography Gel Animals lipids (amino acids peptides and proteins) Lipoproteins HDL Ultracentrifugation Chromatography High Pressure Liquid Triglycerides Lipoprotein |
| Zdroj: | Journal of Veterinary Medical Science. 72:813-817 |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs. |
| Databáze: | OpenAIRE |
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