A CD8 genomic fragment that directs subset-specific expression of CD8 in transgenic mice

Autor:	Hostert, A., Tolaini, M., Festenstein, R., Mcneill, L., Bernard Malissen, Williams, O., Zamoyska, R., Kioussis, D.
Rok vydání:	1997
Předmět:	Transcription Genetic CD8 Antigens Immunology DNA Footprinting Mice Transgenic Thymus Gland Regulatory Sequences Nucleic Acid Mice Inbred C57BL Mice Gene Expression Regulation T-Lymphocyte Subsets Mice Inbred CBA Immunology and Allergy Animals Cloning Molecular Spleen
Zdroj:	Europe PubMed Central Publons
ISSN:	0022-1767
Popis:	Helper and cytotoxic T cell subsets require the expression of different coreceptors (CD4 and CD8, respectively) for their development and function. We have cloned the CD8 gene locus from genomic cosmid and P1 libraries and analyzed the region around the CD8alpha and CD8beta genes for gene expression regulatory elements. DNase I (DNase I) hypersensitivity analysis of 80 kb in the CD8 locus identified four clusters of putative regulatory regions, three of which are thymocyte specific. Transgenic mice carrying the cloned CD8alphabeta genomic locus and containing the identified DNase I-hypersensitive site clusters express the transgenic CD8 in a developmentally regulated, tissue-specific, and CD8 T cell subset-specific manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdfdc92e4d4367d00290893c09029fb1 https://pubmed.ncbi.nlm.nih.gov/9126989 Zobrazit plný text záznamu