Time-resolved studies of energy transfer in thin films of green and red fluorescent proteins
| Autor: | Ifor D. W. Samuel, Joanna M. Zajac, Chang-Min Keum, Thomas Roland, Malte C. Gather, Marcel Schubert |
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| Přispěvatelé: | European Research Council, EPSRC, European Commission, The Royal Society, University of St Andrews. School of Physics and Astronomy, University of St Andrews. Condensed Matter Physics, University of St Andrews. Biomedical Sciences Research Complex |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Energy transfer QH301 Biology 02 engineering and technology Public administration Marie curie Biomaterials 03 medical and health sciences QH301 Time correlated single-photon counting Electrochemistry media_common.cataloged_instance European commission European union Photoluminescence QC media_common Streak camera measurements European research Protein DAS Fluorescent proteins 021001 nanoscience & nanotechnology Condensed Matter Physics T Technology Electronic Optical and Magnetic Materials 030104 developmental biology QC Physics Capital equipment 0210 nano-technology |
| Popis: | This research was financially supported by the European Research Council of the European Union (ERC Grant Agreements No. 640012/ABLASE and 321305/EXCITON), by the Scottish Funding Council (through SUPA), by EPSRC (through the CDT Capital Equipment funding stream, EP/L017008/1) and by a European Commission Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). MS acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (659213). IDWS acknowledges support from a Royal Society Wolfson Research Merit Award. Biologically derived fluorescent proteins are attractive candidates for lasing and sensing due to their excellent optical properties, including their high quantum yield, spectral tunability and robustness against concentration quenching. Here, we report a time-resolved study of the fluorescence dynamics of thin films of Enhanced Green Fluorescent Protein (EGFP), the red-emitting tandem-dimer protein tdTomato and blends of EGFP and tdTomato. We characterized the exciton dynamics by using spectrally and time-resolved measurements of fluorescence and observed a threefold reduction in lifetime when going from solution to thin film, down to 1 ns and 0.6 ns for EGFP and tdTomato, respectively. This finding is attributed to a dipole-dipole non-radiative Förster resonant energy transfer (FRET) in solid-state. We also studied the temporal characteristics of FRET in blended thin films and found increased non-radiative transfer rates. Finally, we report efficient sensitisation of a semiconductor surface with a protein thin film. Such a configuration may have important implications for energy harvesting in hybrid organic-inorganic solar cells and other hybrid optoelectronic devices. Postprint |
| Databáze: | OpenAIRE |
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