Antimicrobial Resistance, Toxinotype, and Genotypic Profiling of Clostridium difficile Isolates of Swine Origin
| Autor: | Wondwossen A. Gebreyes, Pamela R. Fry, Melanie Abley, Siddhartha Thakur |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Genotype Swine Bacterial Toxins Erythromycin Biology medicine.disease_cause Polymerase Chain Reaction Clinical Veterinary Microbiology law.invention Microbiology Feces Antibiotic resistance law Drug Resistance Bacterial North Carolina Pulsed-field gel electrophoresis medicine Animals Humans Polymerase chain reaction Ohio Clostridioides difficile Toxin Sequence Analysis DNA Clostridium difficile Virology Electrophoresis Gel Pulsed-Field Molecular Typing Phylogeography DNA profiling Genes Bacterial medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 50:2366-2372 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.06581-11 |
| Popis: | The occurrence of Clostridium difficile infections in patients that do not fulfill the classical risk factors prompted us to investigate new risk factors of disease. The goal of this study was to characterize strains and associated antimicrobial resistance determinants of C. difficile isolated from swine raised in Ohio and North Carolina. Genotypic approaches used include PCR detection, toxinotyping, DNA sequencing, and pulsed-field gel electrophoresis (PFGE) DNA fingerprinting. Thirty-one percent (37/119) of isolates carried both tetM and tetW genes. The ermB gene was found in 91% of isolates that were resistant to erythromycin (68/75). Eighty-five percent (521/609) of isolates were toxin gene tcdB and tcdA positive. A total of 81% (494/609) of isolates were positive for cdtB and carry a tcdC gene (a toxin gene negative regulator) with a 39-bp deletion. Overall, 88% (196/223) of pigs carry a single C. difficile strain, while 12% (27/223) of pigs carried multiple strains. To the best of our knowledge, this is the first report of individual pigs found to carry more than one strain type of C. difficile . A significant difference in toxinotype profiles in the two geographic locations was noted, with a significantly ( P < 0.001) higher prevalence of toxinotype V found in North Carolina (84%; 189/224) than in Ohio (55%; 99/181). Overall, the study findings indicate that significant proportions of C. difficile in swine are toxigenic and often are associated with antimicrobial resistance genes, although they are not resistant to drugs that are used to treat C. difficile infections. |
| Databáze: | OpenAIRE |
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