Scanning X-ray diffraction on cardiac tissue: automatized data analysis and processing
| Autor: | Tim Salditt, Jan-David Nicolas, Frauke Alves, Manfred Burghammer, M. Andrea Markus, Marten Bernhardt |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Diffraction 030103 biophysics Nuclear and High Energy Physics Materials science 02 engineering and technology Sarcomere Micrometre Protein filament Mice 03 medical and health sciences Data acquisition X-Ray Diffraction Scattering Small Angle medicine Animals Instrumentation Radiation Orientation (computer vision) Cardiac muscle Heart 021001 nanoscience & nanotechnology Cross section (geometry) Crystallography medicine.anatomical_structure 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Synchrotron Radiation. 24:1163-1172 |
| ISSN: | 1600-5775 |
| Popis: | A scanning X-ray diffraction study of cardiac tissue has been performed, covering the entire cross section of a mouse heart slice. To this end, moderate focusing by compound refractive lenses to micrometer spot size, continuous scanning, data acquisition by a fast single-photon-counting pixel detector, and fully automated analysis scripts have been combined. It was shown that a surprising amount of structural data can be harvested from such a scan, evaluating the local scattering intensity, interfilament spacing of the muscle tissue, the filament orientation, and the degree of anisotropy. The workflow of data analysis is described and a data analysis toolbox with example data for general use is provided. Since many cardiomyopathies rely on the structural integrity of the sarcomere, the contractile unit of cardiac muscle cells, the present study can be easily extended to characterize tissue from a diseased heart. |
| Databáze: | OpenAIRE |
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