Comparison of immunoassays for tumor markers CA 19-9, CA 15-3 and CA 125: data from an international quality assessment scheme
| Autor: | Charles Albert Bizollon, Massimo Gion, Alberto Cianetti, Gino Cappelli, Adriano Piffanelli, Emilio Bombardieri, Richard Cohen, Gian Carlo Zucchelli, Alessandro Pilo |
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| Rok vydání: | 1995 |
| Předmět: |
Oncology
Cancer Research medicine.medical_specialty CA-19-9 Antigen International Cooperation CA 15-3 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Internal medicine External quality assessment medicine Humans Antigens Tumor-Associated Carbohydrate Quality of Health Care Immunoassay Quality assessment Mucin-1 General Medicine Prostate-Specific Antigen Carcinoembryonic Antigen Italy 030220 oncology & carcinogenesis CA-125 Antigen Environmental science Regression Analysis CA19-9 Immunoradiometric Assay alpha-Fetoproteins |
| Zdroj: | Tumori. 81(2) |
| ISSN: | 0300-8916 |
| Popis: | Data collected in the 1993 and 1994 cycles of an international external quality assessment (EQA) program and in a national multicenter collaborative study were cumulatively analyzed to evaluate the standardization of the methods currently in use for the assay of mucinous tumor markers CA 19-9, CA 15-3 and CA 125. On average the between-laboratory variability was 15.2 and 16.0 CV% for CA 15-3 and CA 125 respectively; the between-laboratory variability found for CA 19-9 was markedly worse (mean 28.3 CV%). The variability component attributable to systematic differences between different methods/kits was relatively small for CA 15-3 and CA 125 (18% and 24% of the total variability) but markedly larger for CA 19-9 (48% of the total variability). The agreement of CA 19-9 results worsened in the last few years when new nonisotopic techniques became available. The precision of the methods/kits most used in the survey ranged from 9.9 to 13.3 CV% for CA 125 and from 11.6 to 13.9 CV% for CA 15-3. For these two tumor markers the precision of the traditional IRMAs does not appear different from that of the new fully automated nonisotopic techniques. The precision of CA 19-9 methods was on average worse (from 11.7 to 19.6 CV%) although two automated systems exhibited a precision better than that of IRMAs. In conclusion, the results of this study indicate that CA 15-3 and CA 125 are satisfactorily assayed whereas CA 19-9 assay appears affected by larger differences between methods and by poorer precision of laboratories and kits. |
| Databáze: | OpenAIRE |
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