Carbon-13 Cross-Polarization Magic-Angle Spinning Nuclear Magnetic Resonance for Measuring Proanthocyanidin Content and Procyanidin to Prodelphinidin Ratio in Sainfoin (Onobrychis viciifolia) Tissues
| Autor: | Irene Mueller-Harvey, Honorata M. Ropiak, Lydia M.J. Smith, Radoslaw M. Kowalczyk, Christos Fryganas, Marina Mora-Ortiz, Chris Drake |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Magnetic Resonance Spectroscopy 01 natural sciences Catechin chemistry.chemical_compound Magic angle spinning Biflavonoids Lignin Proanthocyanidins Carbon Isotopes Chromatography biology Plant Extracts Cross polarization 010401 analytical chemistry Carbon-13 Onobrychis viciifolia food and beverages Fabaceae General Chemistry biology.organism_classification 0104 chemical sciences chemistry Thiolysis Proanthocyanidin General Agricultural and Biological Sciences Prodelphinidin 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:4073-4081 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.8b01215 |
| Popis: | A procedure based on 13C CPMAS NMR was developed to study procyanidins (PCs) and prodelphinidins (PDs) directly in milled sainfoin plant tissues. Blackcurrant and Tilia samples enabled reference spectra of purified proanthocyanidin (PA) fractions, crude extracts, and milled plant tissues, with characteristic resonances at 155, 144, and 132 ppm. PC/PD ratios were estimated from the I132/I155 intensity ratio and differed by 2.5 to 5.9% compared to thiolysis data. Normalization to the 155 ppm signal intensity from reference spectra enabled analysis of PA contents with an error of ca. 8 g PAs/100 g plant tissue. The procedure estimates the lignin contribution and allows for a correction of the PA content. In six sainfoin accessions, estimated PA contents were 1.6- to 20.8-fold higher than the thiolysis and 1.4- to 2.6-fold higher than the HCl–butanol–acetone results. Method differences may reflect the presence of unextractable, possibly high molecular weight PAs in sainfoin. |
| Databáze: | OpenAIRE |
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