Evaluation of Immunophenotypic and Molecular Biomarkers for Sézary Syndrome Using Standard Operating Procedures: A Multicenter Study of 59 Patients
| Autor: | Anne Marie-Cardine, Renata Ponti, Stephanie E. Boonk, Laurence Michel, Jacoba J. Out-Luiting, Moritz Felcht, Maria Grazia Bernengo, Rein Willemze, Emanuela Barberio, Leslie van der Fits, Tracey J. Mitchell, Kirsi Jäntti, Isabella Tosi, Maarten H. Vermeer, Caroline Ram-Wolff, Sean Whittaker, Annamari Ranki, Claus-Detlev Klemke, Blaithin Moriarty, Cornelis P. Tensen, Nina Booken, Armand Bensussan, S L Morris, Martine Bagot, Willem H. Zoutman, Pietro Quaglino |
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| Přispěvatelé: | Department of Dermatology, Allergology and Venereology, Clinicum |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Pathology Gene Dosage EUROPEAN-ORGANIZATION Polymerase Chain Reaction Biochemistry T-CELL LYMPHOMA 030207 dermatology & venereal diseases 0302 clinical medicine T-cell lymphoma STAT4 Aged 80 and over FLOW-CYTOMETRY ABERRANT EXPRESSION FOR-CUTANEOUS-LYMPHOMAS Middle Aged Flow Cytometry MYCOSIS-FUNGOIDES 3. Good health Europe Real-time polymerase chain reaction 030220 oncology & carcinogenesis EORTC CLASSIFICATION Female Adult medicine.medical_specialty ERYTHRODERMIC PATIENTS Dermatology PERIPHERAL-BLOOD Sensitivity and Specificity Skin Diseases Peripheral blood mononuclear cell Gene dosage Immunophenotyping Diagnosis Differential 03 medical and health sciences medicine Humans Sezary Syndrome Molecular Biology Sezary Cell Aged Inflammation Mycosis fungoides INTERFERON-GAMMA business.industry Gene Expression Profiling Reproducibility of Results Cell Biology medicine.disease Gene Expression Regulation 3121 General medicine internal medicine and other clinical medicine Differential diagnosis business Biomarkers |
| Zdroj: | Boonk, S E, Zoutman, W H, Marie-Cardine, A, van der Fits, L, Out-Luiting, J J, Mitchell, T J, Tosi, I, Morris, S L, Moriarty, B, Booken, N, Felcht, M, Quaglino, P, Ponti, R, Barberio, E, Ram-Wolff, C, Jäntti, K, Ranki, A, Bernengo, M G, Klemke, C-D, Bensussan, A, Michel, L, Whittaker, S, Bagot, M, Tensen, C P, Willemze, R & Vermeer, M H 2016, ' Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures: multicenter study of 59 cases ', Journal of Investigative Dermatology . https://doi.org/10.1016/j.jid.2016.01.038 Journal of Investigative Dermatology, 136(7), 1364-1372. ELSEVIER SCIENCE INC |
| DOI: | 10.1016/j.jid.2016.01.038 |
| Popis: | Differentiation between Sezary syndrome and erythrodermic inflammatory dermatoses can be challenging, and a number of studies have attempted to identify characteristic immunophenotypic changes and molecular biomarkers in Sezary cells that could be useful as additional diagnostic criteria. In this European multicenter study, the sensitivity and specificity of these immunophenotypic and recently proposed but unconfirmed molecular biomarkers in Sezary syndrome were investigated. Peripheral blood CD4 + T cells from 59 patients with Sezary syndrome and 19 patients with erythrodermic inflammatory dermatoses were analyzed for cell surface proteins by flow cytometry and for copy number alterations and differential gene expression using custom-made quantitative PCR plates. Experiments were performed in duplicate in two independent centers using standard operating procedures with almost identical results. Sezary cells showed MYC gain (40%) and MNT loss (66%); up-regulation of DNM3 (75%), TWIST1 (69%), EPHA4 (66%), and PLS3 (66%); and down-regulation of STAT4 (91%). Loss of CD26 (≥80% CD4 + T cells) and/or CD7 (≥40% CD4 + T cells) and combination of altered expression of STAT4 , TWIST1 , and DNM3 or PLS3 could distinguish, respectively, 83% and 98% of patients with Sezary syndrome from patients with erythrodermic inflammatory dermatoses with 100% specificity. These additional diagnostic panels will be useful adjuncts in the differential diagnosis of Sezary syndrome versus erythrodermic inflammatory dermatoses. |
| Databáze: | OpenAIRE |
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