The AeAct-4 gene is expressed in the developing flight muscles of female Aedes aegypti
| Autor: | Delia Muñoz, Anthony A. James, Osvaldo Marinotti, Alyssa J. Jimenez |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Aedes aegypti Biology Dengue fever Aedes parasitic diseases Genetics medicine Gene family Animals Cluster Analysis Amino Acid Sequence Muscle Skeletal Molecular Biology Gene In Situ Hybridization Phylogeny DNA Primers Gene Library Base Sequence cDNA library Gene Expression Profiling Yellow fever fungi Pupa Promoter Sequence Analysis DNA pupal expression biology.organism_classification medicine.disease Blotting Northern Virology Actins Gene expression profiling Blotting Southern Gene Components muscle actin Insect Science Flight Animal RIDL Female female-specific Sequence Alignment |
| Zdroj: | Muñoz, Delia; Jimenez, Alyssa J.; Marinotti, Osvaldo; & James, Anthony A.(2004). The AeAct-4 gene is expressed in the developing flight muscles of female Aedes aegypti. Insect Molecular Biology, 13(5), 563-568. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/7r5687cp |
| Popis: | Population reduction of mosquitoes is an effective method for controlling dengue fever and malaria transmission. Recent developments in control techniques include proposals to construct transgenic strains of mosquitoes carrying dominant, conditional-lethal genes under the control of sex- and stage-specific promoters. In order to identify such promoters, subtractive cDNA libraries derived from male and female pupal mRNA of the yellow fever mosquito, Aedes aegypti, were constructed and screened. A cDNA clone, F49, corresponds to a gene expressed specifically in female pupae. Sequence analyses revealed that this gene belongs to the actin gene family, and therefore was designated Aedes Actin-4 (AeAct-4). Transcription analyses demonstrated that this gene is expressed predominantly in the indirect flight muscles and, to a lesser extent, the legs of developing female mosquitoes. The promoter of this gene may be a useful tool for developing conditional lethal strains of mosquitoes. |
| Databáze: | OpenAIRE |
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