Effects of exogenously-applied L-ascorbic acid on root expansive growth and viability of the border-like cells
| Autor: | Min Yu, Veranika Samokhina, Maryia Makavitskaya, V. Mackievic, Vadim Demidchik, I. Navaselsky, Li Xuewen, Sergey Shabala, Galina Smolikova, Sergei Medvedev, P. Hryvusevich |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Ascorbic Acid Plant Science Plant Roots 01 natural sciences Pisum 03 medical and health sciences chemistry.chemical_compound Border cells Arabidopsis thaliana Calcium Signaling Propidium iodide chemistry.chemical_classification Reactive oxygen species biology food and beverages Ascorbic acid biology.organism_classification Article Addendum Cytosol 030104 developmental biology chemistry Biophysics Elongation Reactive Oxygen Species 010606 plant biology & botany |
| Zdroj: | Plant Signaling & Behavior. 13:e1514895 |
| ISSN: | 1559-2324 |
| DOI: | 10.1080/15592324.2018.1514895 |
| Popis: | Functions of exogenous L-ascorbic acid in plant roots are poorly understood. Recent study by Makavitskaya et al. (doi.org/10.1093/jxb/ery056) has demonstrated that exogenous ascorbate can be released from roots in response to salt stress, and can trigger elevation in the cytosolic free Ca(2+). Here, we report that exogenous ascorbate significantly modifies root elongation in Arabidopsis thaliana. Using a medium exchange technique, we have shown that 10–100 µM ascorbate induces small but significant increase in root elongation while higher levels cause its dramatic decrease. Root border cells of Pisum sativum have been losing viability twice faster in the presence of ascorbate that under control conditions, as tested by the confocal microscopy and a combined staining with propidium iodide and fluorescein diacetate. |
| Databáze: | OpenAIRE |
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