Expression and modulation by cytokines of the intercellular adhesion molecule-1 (ICAM-1) in human central nervous system tumor cell cultures
| Autor: | Jeffrey N. Bruce, Ludovico Guarini, Paul B. Fisher, Soldano Ferrone, Arthur P. Bollon, Thomas A. Moulton, Gregory J. Duigou, Massimo Temponi |
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| Rok vydání: | 1990 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Time Factors medicine.drug_class Intercellular Adhesion Molecule-1 Fluorescent Antibody Technique Biology Monoclonal antibody Interferon-gamma Immune system Downregulation and upregulation medicine Tumor Cells Cultured Humans ICAM-1 Brain Neoplasms Tumor Necrosis Factor-alpha Antibodies Monoclonal Intercellular adhesion molecule Recombinant Proteins Oncology Cell culture Interferon Type I Cancer research Tumor necrosis factor alpha Cell Adhesion Molecules |
| Zdroj: | International journal of cancer. 46(6) |
| ISSN: | 0020-7136 |
| Popis: | The intercellular adhesion molecule (ICAM-1) has been shown to be important in interactions involving cells of the immune system and to be upregulated in a number of cell culture systems by cytokines, including immune interferon (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). In the present study, we have determined by fluorescence-activated cell sorter (FACS) analysis and the anti-ICAM-1 monoclonal antibody (MAb) CL203.4 the base-line expression of ICAM-1 and its modulation by recombinant IFN-beta, IFN-gamma and TNF-alpha in early passage (less than 15) human central nervous system (CNS) tumor-derived cell cultures. These cultures were established from various malignancies, including glioblastoma multiforme (GBM), astrocytoma (AST), ganglioglioma, medulloblastoma, meningioma and a pineal tumor. ICAM-1 expression was highest in the GBM- and AST-derived cell cultures and was lowest in the ganglioglioma and normal pineal cell cultures. Variable ICAM-1 expression was found, however, in tumors of the same histological group. In several cell cultures the variable expression observed by FACS was substantiated by the intensity of the molecular species immunoprecipitated by the anti-ICAM-1 MAb CL203.4 from these cells. All the cell cultures displayed variable but consistent increases in ICAM-1 expression following treatment with IFN-gamma or TNF-alpha. In general, the degree of increase in ICAM-1 expression was greatest in cultures exposed to TNF-alpha. Upregulation of ICAM-1 expression in an established glioblastoma multiforme cell line was of greater magnitude and more rapid following TNF-alpha treatment (within 2 to 3 hr) than exposure to IFN-gamma (by 24 hr). In several cultures, IFN-beta also increased ICAM-1 expression and enhanced the increase induced by TNF-alpha. The results of the present study indicate that variable expression of ICAM-1 is a common property of early passage cultures of CNS tumors and recombinant interferons and TNF-alpha can differentially upregulate ICAM-1 expression in these CNS tumor cell cultures. |
| Databáze: | OpenAIRE |
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