Trigonella foenum-graecum Seeds Oil Attenuated Inflammation and Angiogenesis in vivo through Down-Regulation of TNF-α
| Autor: | Tahir Ali Chohan, Mahrukh, Hafiz Muhammad Yousaf, Mohammed Saleem, Malik Saadullah, Hafiza Sidra Yaseen, Muhammad Usman Shamas, Muhammad Umair Yousaf, Maria Yaseen, Muhammad Asif |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Staphylococcus aureus Cancer Research Antifungal Agents Antioxidant Trigonella medicine.medical_treatment Phytochemicals Anti-Inflammatory Agents Down-Regulation Angiogenesis Inhibitors Microbial Sensitivity Tests Pharmacology Superoxide dismutase Structure-Activity Relationship chemistry.chemical_compound In vivo Candida albicans Staphylococcus epidermidis medicine Animals Edema Plant Oils Gallic acid Inflammation Granuloma Dose-Response Relationship Drug Molecular Structure Neovascularization Pathologic biology Tumor Necrosis Factor-alpha Chemistry biology.organism_classification Anti-Bacterial Agents Rats Carrageenan Phytochemical Seeds biology.protein Molecular Medicine Female Heptadecanoic acid Aspergillus niger |
| Zdroj: | Anti-Cancer Agents in Medicinal Chemistry. 21:1460-1471 |
| ISSN: | 1871-5206 |
| Popis: | Introduction: Inflammation is a vital reaction of the natural immune system that protects against encroaching agents. However, uncontrolled inflammation can lead to complications. Trigonella foenumgraecum is traditionally used as an anti-inflammatory herb. Objectives: The current study was conducted to explore the antioxidant, anti-inflammatory, and antiangiogenic potentials of Trigonella foenum-graecum seeds oil. Methods: Oil was extracted from seeds of Trigonella foenum-graecum by cold press method and labelled as TgSO. Phytochemical (GC-MS, Folin-Ciocalteu method) and metal analyses were conducted to evaluate the metalo-chemical profile of TgSO. In vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3- ethylbenzothiazoline-6-sulfonic acid and ferric reducing antioxidant power) were performed to assess its antioxidant potential. In vitro antimicrobial activity was evaluated using agar disc diffusion method and the safety profile of TgSO was assessed in acute toxicological studies following OECD 425 guidelines. In vivo antiinflammatory activities of TgSO were assessed in carrageenan, serotonin, histamine, formalin, and cotton pelletinduced oedema models. Serum TNF-α, Superoxide Dismutase (SOD) and, Catalases (CAT) levels were assessed by ELISA kits. In vivo antiangiogenic activity of TgSO was screened in chick Chorioallantoic Membrane (CAM) assay. Histopathological studies using excised paws were conducted to observe the effects of TgSO treatment at the tissue level. In silico docking studies were conducted to screen the binding potentials of identified compounds with TNF-α. Results: Extraction by cold press method yielded 16% of TgSO. Phytochemical analysis of TgSO through GCMS showed the presence of eugenol, dihydrocoumairn, heptadecanoic acid, tri- and tetradecanoic acid, and hexadecanoic acid, respectively. Total phenolic contents of TgSO were found to be 0.30±0.01mg/g gallic acid equivalent in Folin-Ciocalteu method. Metal analysis indicated the presence of different metals in TgSO. Findings of antioxidant models showed the moderate antioxidant potential of TgSO. Findings of antimicrobial assays showed that TgSO was active against bacterial (S. aureus, S. epidermidis) and fungal (C. albicans, and A. niger) strains. In vivo toxicity study data showed that TgSO was safe up to the dose of 5000 mg/kg. Data of oedema models showed a significant (p Conclusion: Data obtained from the current study conclude that TgSO has antioxidant, anti-inflammatory, and antiangiogenic effects that validate its traditional uses. Synergistic actions of different phytoconstituents are proposed to be responsible for the observed effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|