Comparison of Molecular Subtyping and Antimicrobial Resistance Detection Methods Used in a Large Multistate Outbreak of Extensively Drug-Resistant Campylobacter jejuni Infections Linked to Pet Store Puppies
| Autor: | Scott Robertson, Lavin A. Joseph, Rachel Silver, Mark E. Laughlin, Cindy R. Friedman, Lauren Stevenson, Janet M. Pruckler, Hayat Caidi, Megin Nichols, Christy Bennett, Heather A. Carleton, Kelley Hise, Jessica C. Chen, Lia Koski, Colin Basler, Rachael D. Aubert, Kaitlin A. Tagg, Hannes Pouseele, Aimee L. Geissler, Jason P. Folster, Louise Francois Watkins |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Genotype Epidemiology Drug resistance Biology medicine.disease_cause Campylobacter jejuni Disease Outbreaks Dogs Campylobacter Infections Drug Resistance Bacterial medicine Pulsed-field gel electrophoresis Animals Humans Genetics Molecular epidemiology Campylobacter Outbreak biology.organism_classification Subtyping Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Pharmaceutical Preparations Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | J Clin Microbiol |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Campylobacter jejuni is a leading cause of enteric bacterial illness in the United States. Traditional molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE) and 7-gene multilocus sequence typing (MLST), provided limited resolution to adequately identify C. jejuni outbreaks and separate out sporadic isolates during outbreak investigations. Whole-genome sequencing (WGS) has emerged as a powerful tool for C. jejuni outbreak detection. In this investigation, 45 human and 11 puppy isolates obtained during a 2016–2018 outbreak linked to pet store puppies were sequenced. Core genome multilocus sequence typing (cgMLST) and high-quality single nucleotide polymorphism (hqSNP) analysis of the sequence data separated the isolates into the same two clades containing minor within-clade differences; however, cgMLST analysis does not require selection of an appropriate reference genome, making the method preferable to hqSNP analysis for Campylobacter surveillance and cluster detection. The isolates were classified as sequence type 2109 (ST2109)—a rarely seen MLST sequence type. PFGE was performed on 38 human and 10 puppy isolates; PFGE patterns did not reliably predict clustering by cgMLST analysis. Genetic detection of antimicrobial resistance determinants predicted that all outbreak-associated isolates would be resistant to six drug classes. Traditional antimicrobial susceptibility testing (AST) confirmed a high correlation between genotypic and phenotypic antimicrobial resistance determinations. WGS analysis linked C. jejuni isolates in humans and pet store puppies even when canine exposure information was unknown, aiding the epidemiological investigation during the outbreak. WGS data were also used to quickly identify the highly drug-resistant profile of these outbreak-associated C. jejuni isolates. |
| Databáze: | OpenAIRE |
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