Lens epithelial apoptosis and cell proliferation in human age-related cortical cataract
| Autor: | G Delides, Dimitrios Brouzas, Antonios Charakidas, Pavlos Koukoulomatis, Alexandra Kalogeraki, Miltiadis K. Tsilimbaris |
|---|---|
| Předmět: |
Adult
Male Aging genetic structures medicine.medical_treatment Population Lens Capsule Crystalline Apoptosis Cataract Extraction Biology Cataract Andrology 03 medical and health sciences 0302 clinical medicine Age-related cortical cataract In Situ Nick-End Labeling medicine Humans Proliferation Marker education Aged Cell Proliferation Aged 80 and over education.field_of_study TUNEL assay Cell growth Cell Cycle Antibodies Monoclonal Epithelial Cells DNA Lens Cortex Crystalline General Medicine Anatomy Middle Aged Cell cycle eye diseases Ophthalmology Ki-67 Antigen Antibodies Antinuclear 030221 ophthalmology & optometry Capsulotomy Female sense organs 030217 neurology & neurosurgery |
| Zdroj: | Scopus-Elsevier |
| Popis: | PurposeTo probe the presence of apoptosis in the epithelium of human lenses with age-related cortical cataract as well as to assess cell proliferation, a predicted consequence of apoptotic cell death, in this specific cell population.MethodsDNA fragmentation was assessed using terminal digoxigenin-labeled dUTP nick end labeling (TUNEL) in capsulotomy specimens obtained from patients who underwent either extracapsular cataract extraction for the removal of adult-onset cortical cataract (n=27) or clear lens extraction for the correction of high myopia (n=25). Cell proliferation was assayed in 23 epithelia of cataractous lenses, and 20 epithelia of non-cataractous lenses with the proliferation marker MIB1, a monoclonal antibody against the nuclear antigen Ki-67 that is detected throughout the cell cycle but is absent in the resting (G0) cell.ResultsTUNEL staining was observed in 25 (92.6%) specimens of cataractous lenses, whereas cells undergoing apoptosis were identified in 2 (8%) of the epithelia from non-catarac-tous lenses. Only two MIB1-positive samples were detected, one of which was a capsule obtained during intracapsular cataract extraction.ConclusionsThe epithelium of human lenses with cortical cataract undergoes low rate apoptotic death. This limited epithelial apoptosis is unlikely to result in any significant cell density decrease since epithelial gaps are likely to be replaced by cell proliferation at the germinative zone of the anterior lens capsule. Nevertheless, the accumulation of small-scale epithelial losses during lifetime may induce alterations in lens fiber formation and homeostasis and result in loss of lens transparency. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|