| Autor: |
Pieter Deschaght, T.N. Kiama, Marleen Temmerman, Kimberly K. Jefferson, Hans Verstraelen, Nabil Abdullah El Aila, Mario Vaneechoutte, Guido Lopes dos Santos Santiago |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
American Journal of Obstetrics and Gynecology. 204:450.e1-450.e7 |
| ISSN: |
0002-9378 |
| DOI: |
10.1016/j.ajog.2010.12.061 |
| Popis: |
Objective Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase. Study Design One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test. Results Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase. Conclusion Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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