Gender-Specific Toxicological Effects of Chronic Exposure to Pure Microcystin-LR or Complex Microcystis aeruginosa Extracts on Adult Medaka Fish
| Autor: | Chakib Djediat, Gérard Bolbach, Marc Edery, Séverine Le Manach, Gilles Clodic, Benjamin Marie, Cécile Bernard, Qin Qiao, Hélène Huet, Nour Khenfech, Arul Marie, Charlotte Duval |
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| Přispěvatelé: | Molécules de Communication et Adaptation des Micro-Organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire d'Alfort (ENVA), Spectrométrie de Masse et Protéomique [IBPS] (IBPS-SPM), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cyanobacteria Proteomics Microcystis Microcystins Oryzias Microcystin-LR 010501 environmental sciences 01 natural sciences Oogenesis Microbiology 03 medical and health sciences chemistry.chemical_compound medicine Environmental Chemistry Animals [CHIM]Chemical Sciences Microcystis aeruginosa 14. Life underwater 0105 earth and related environmental sciences biology Glycogen Hepatotoxin General Chemistry biology.organism_classification 6. Clean water Lakes 030104 developmental biology medicine.anatomical_structure chemistry 13. Climate action Hepatocyte |
| Zdroj: | Environmental Science and Technology Environmental Science and Technology, American Chemical Society, 2016, 50 (15), pp.8324-8334. ⟨10.1021/acs.est.6b01903⟩ Environmental Science and Technology, 2016, 50 (15), pp.8324-8334. ⟨10.1021/acs.est.6b01903⟩ |
| ISSN: | 0013-936X 1520-5851 |
| DOI: | 10.1021/acs.est.6b01903⟩ |
| Popis: | International audience; Cyanobacterial blooms often occur in freshwater lakes and constitute a potential health risk to human populations, as well as to other organisms. However, their overall and specific implications for the health of aquatic organisms that are chronically and environmentally exposed to cyanobacteria producing hepatotoxins, such as microcystins (MCs), together with other bioactive compounds have still not been clearly established and remain difficult to assess. The medaka fish was chosen as the experimental aquatic model for studying the cellular and molecular toxicological effects on the liver after chronic exposures (28 days) to environmentally relevant concentrations of pure MC-LR, complex extracts of MC producing or nonproducing cyanobacterial biomasses, and of a Microcystis aeruginosa natural bloom. Our results showed a higher susceptibility of females to the different treatments compared to males at both the cellular and the molecular levels. Although hepatocyte lysis increased with MC-containing treatments, lysis always appeared more severe in the liver of females compare to males, and the glycogen cellular reserves also appeared to decrease more in the liver of females compared to those in the males. Proteomic investigations reveal divergent responses between males and females exposed to all treatments, especially for proteins involved in metabolic and homeostasis processes. Our observations also highlighted the dysregulation of proteins involved in oogenesis in female livers. These results suggest that fish populations exposed to cyanobacteria blooms may potentially face several ecotoxicological issues. |
| Databáze: | OpenAIRE |
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