Measurement of Interleukin-6 Production by Monocytes for in Vitro Safety Testing of Hemoglobin Solutions
| Autor: | P. J. Den Boer, J. C. Bakker, E. M. De Groot, Wim K. Bleeker, P. T. M. Biessels, L. A. Aarden |
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| Rok vydání: | 1994 |
| Předmět: |
Fever
Biomedical Engineering Inflammation In Vitro Techniques Biology Monocytes Blood substitute Blood cell Hemoglobins Blood Substitutes medicine Humans Acute-Phase Reaction Interleukin-6 Monocyte Acute-phase protein biology.organism_classification In vitro Endotoxins Solutions medicine.anatomical_structure Biochemistry Limulus Hemoglobin Safety medicine.symptom Biotechnology |
| Zdroj: | Artificial Cells, Blood Substitutes, and Biotechnology. 22:835-840 |
| ISSN: | 1532-4184 1073-1199 |
| DOI: | 10.3109/10731199409117918 |
| Popis: | Induction of interleukin-6 (IL-6) production by isolated human mononuclear blood cells was taken as in vitro model for the induction of inflammatory reactions. The model was very sensitive to bacterial endotoxin (detection limit < 10pg/ml). Hemoglobin (Hb) solutions, prepared under non-sterile conditions also induced IL-6 production, which correlated with a positive reaction in the Limulus assay. Purification of the Hb solutions with a detergent prevented IL-6 production, showing that pure Hb itself does not activate the monocytes. We conclude that this assay is a useful and sensitive test of contamination with components that can induce inflammatory reactions, especially microbial products. |
| Databáze: | OpenAIRE |
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