Coexistence of SFO-1 and NDM-1 β-lactamase genes and fosfomycin resistance gene fosA3 in an Escherichia coli clinical isolate
| Autor: | Cha Xu, Jing-yi Zhao, Xiao-dong Mu, Yan-nian Li, Li-ping You, Ping Qin, Jin-long Ma, Yuan-qi Zhu |
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| Rok vydání: | 2015 |
| Předmět: |
China
Gene Transfer Horizontal Drug resistance Fosfomycin medicine.disease_cause Microbiology beta-Lactamases Plasmid Antibiotic resistance Drug Resistance Bacterial Escherichia coli Genetics medicine Humans Molecular Biology Escherichia coli Infections biology Middle Aged biology.organism_classification Antimicrobial Enterobacteriaceae Anti-Bacterial Agents New Delhi metallo-beta-lactamase 1 Genes Bacterial Conjugation Genetic biology.protein Female Plasmids medicine.drug |
| Zdroj: | FEMS Microbiology Letters. 362:1-7 |
| ISSN: | 1574-6968 |
| DOI: | 10.1093/femsle/fnu018 |
| Popis: | This study aims to characterize antimicrobial resistance and antimicrobial resistance genetic determinants of an Escherichia coli clinical isolate HD0149 from China in 2012. This strain displayed high-level resistance to cephalosporins, carbapenems, fluoroquinolones, aminoglycosides and fosfomycin. A range of antimicrobial resistance genes was detected responsible for its multiple antimicrobial resistances, involving the blaCMY-2, blaCTX-M-65, blaNDM-1, blaSFO-1, blaTEM-1, fosA3, rmtB, sul1 and sul2 genes. Four amino acid substitutions were detected in the quinolone resistance-determining regions (QRDRs) of GyrA (S83L and D87N), ParC (S80I) and ParE (S458A). Conjugation experiments revealed two multiresistance plasmids present in E. coli HD0149. The blaSFO-1 gene associated with blaNDM-1 gene was located in a 190 kb IncA/C plasmid and the blaCTX-M-65, fosA3 and rmtB genes were located in a 110 kb IncF plasmid. This is the first identification of the blaSFO-1 gene in an E. coli isolate and on a conjugative IncA/C plasmid. This may dramatically enhance the international prevalence and dissemination of blaSFO-1 among Enterobacteriaceae. |
| Databáze: | OpenAIRE |
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