Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice
| Autor: | Takafumi Tezuka, Mitsunori Hayashi, Jirong Huang, Maki Kawai-Yamada, Hirofumi Uchimiya, Hideyuki Takahashi, Li-Hua Yu, Katsunori Tamura |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Niacinamide
Programmed cell death Time Factors Recombinant Fusion Proteins Reductase Models Biological Zea mays Mass Spectrometry Cofactor Amide Synthases Homeostasis Cochliobolus carbonum Coloring Agents Glutathione Transferase Ions chemistry.chemical_classification Multidisciplinary Bacterial disease Cell Death biology Electrophoresis Capillary food and beverages Oryza Hydrogen-Ion Concentration Biological Sciences NAD Plants Genetically Modified biology.organism_classification Genetically modified rice Plant Leaves Alcohol Oxidoreductases Phosphotransferases (Alcohol Group Acceptor) Enzyme chemistry Biochemistry biology.protein NAD+ kinase Reactive Oxygen Species NADP Evans Blue |
| Zdroj: | ResearcherID |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The maize Hm1 gene encoding the NADPH-dependent HC-toxin reductase is capable of detoxifying HC-toxin of fungus Cochliobolus carbonum . Here, we conducted the metabolic and biochemical analysis in transgenic rice plants overexpressing an HC-toxin reductase-like gene in rice ( YK1 gene). Methods employing NADPH oxidation and capillary electrophoresis mass spectrometry analysis confirmed that YK1 possessed dihydroflavonol-4-reductase activity in vitro and in vivo . The overexpression of YK1 in both suspension-cultured cells and rice plants increased NAD(H) and NADP(H) levels by causing an increase in NAD synthetase and NAD kinase activities. Activity changes in enzymes that require NAD(P) as coenzymes were also noted in rice cells ectopically expressing YK1, where the cell death caused by hydrogen peroxide and bacterial disease was down-regulated. Thus, a strategy was proposed that the combination of dihydroflavonol-4-reductase activity and the elevated level of NAD(P)H pool may confer the prevention of induced cell death in planta . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|