Ubiquitin gene expression: response to environmental changes
| Autor: | Reginald Storms, Jeana Neculcea, Judith Fraser, David Y. Thomas, Hue Anh Luu |
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| Rok vydání: | 1991 |
| Předmět: |
Hot Temperature
Deoxyribonucleotides Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Adaptation Biological Biology medicine.disease_cause Ubiquitin Gene Expression Regulation Fungal Gene expression Genetics medicine Ubiquitins Gene Mutation Base Sequence Cell growth Cell Cycle General Medicine Cell cycle Methyl Methanesulfonate biology.organism_classification Molecular biology 4-Nitroquinoline-1-oxide Yeast Culture Media Cell biology biology.protein |
| Zdroj: | Current Genetics. 20:17-23 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00312760 |
| Popis: | It has previously been shown that the yeast ubiquitin genes UBI1, 2 and 3 are strongly expressed during the log-phase of batch culture growth, whereas the UBI4 gene is weakly expressed. We found that heat shock, treatment with DNA-damaging agents, starvation, and the feeding of starved cells all transiently induced UBI4. These results suggest that UBI4 is induced whenever a change in culture conditions dictates a dramatic shift in cellular metabolism, and that UBI4 expression returns to lower levels once cellular metabolism has adapted to the new conditions. In contrast, all of the treatments tested, except starvation, transiently repressed the UBI1, 2 and 3 genes. Although starvation also repressed UBI1, 2 and 3 its effect was not transient, and expression only recovered upon the addition of fresh media. These results, together with others presented here, suggest that high levels of UBI1, 2 and 3 expression are dependant upon ongoing cell growth, and that treatments which slow or stop growth repress their expression. |
| Databáze: | OpenAIRE |
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