| Autor: |
Geiselman, Gina M., Kirby, James, Landera, Alexander, Otoupal, Peter, Papa, Gabriella, Barcelos, Carolina, Sundstrom, Eric R., Lalitendu Das, Harsha D. Magurudeniya, Wehrs, Maren, Rodriguez, Alberto, Simmons, Blake A., Magnuson, Jon K., Aindrila Mukhopadhyay, Lee, Taek Soon, Anthe George, Gladden, John M. |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.13233201.v1 |
| Popis: |
Additional file 1: Table S1. All metabolites detected by GC-MS from (A) prespatane synthase and (B) epi-isozizaene synthase expressed in R. toruloides. Relative abundances were approximated by corrected peak area. Molecules have not been confirmed with standards and tentative molecule assignments are based on mass spectrum matches. Table S2. Relative reliability of the viscosity methods SUPERTRAPP and Pedersen (ªD, Absolute Average Deviation) for molecules included in the blend model. Table S3. Concentration of compounds tracked by HPLC in batch 2 poplar hydrolysate supplemented with 5 g/L ammonium sulfate. Figure S1. Viscosity blending behavior of saturated prespatane and saturated epi-isozizaene at a − 20 °C and b − 40 °C. Figure S2. Liquid density blending behavior of saturated prespatane and saturated epi-isozizaene at 15 °C. Figure S3. Viscosities of saturated prespatane and saturated epi-isozizaene, in the temperature range of − 40–40 °C. Figure S4. Sesquiterpene titers of the highest terpene producing strain for each construct shown in Fig. 2 before stacking of HYG and NAT constructs. Figure S5. Nitrogen source supplementation comparisons in poplar hydrolysate. Figure S6. The initial 2 L fermentation run resulted in a low prespatane titer, which was attributed to a possible magnesium and phosphate deficiency. Figure S7. Organic acids from PPS5 fermentation with unfiltered hydrolysate batch 3. Figure S8. Prespatane production by PPS5 grown in filtered and unfiltered poplar hydrolysate. Figure S9. Validation of a viscosity model for Jet A. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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