Waddlia chondrophila induces systemic infection, organ pathology, and elicits Th1-associated humoral immunity in a murine model of genital infection
| Autor: | Vasilevsky, Sam, Gyger, Joel, Piersigilli, Alessandra, Pilloux, Ludovic, Greub, Gilbert, Stojanov, Milos, Baud, David |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Chlamydiales
630 Agriculture genital tract infection intracellular bacteria lcsh:QR1-502 zoonosis Th1 Cells Microbiology Antibodies Bacterial Immunohistochemistry Reproductive Tract Infections lcsh:Microbiology Immunity Humoral Chlamydia-like Disease Models Animal Mice Liver Immunoglobulin G Animals Antibodies Bacterial/blood Chlamydiales/physiology Gram-Negative Bacterial Infections/microbiology Gram-Negative Bacterial Infections/pathology Immunoglobulin G/blood Liver/microbiology Liver/pathology Lymph Nodes/microbiology Reproductive Tract Infections/microbiology Reproductive Tract Infections/pathology Spleen/microbiology Spleen/pathology Th1 Cells/immunology Lymph Nodes adverse pregnancy outcome Gram-Negative Bacterial Infections Spleen Original Research |
| Zdroj: | Frontiers In Cellular and Infection Microbiology, vol. 5, pp. 76 Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, Vol 5 (2015) Vasilevsky, Sam; Gyger, Joel; Piersigilli, Alessandra; Pilloux, Ludovic; Greub, Gilbert; Stojanov, Milos; Baud, David (2015). Waddlia chondrophila induces systemic infection, organ pathology, and elicits Th1-associated humoral immunity in a murine model of genital infection. Frontiers in cellular and infection microbiology, 5, p. 76. Frontiers 10.3389/fcimb.2015.00076 |
| DOI: | 10.3389/fcimb.2015.00076 |
| Popis: | Waddlia chondrophila is a known bovine abortigenic Chlamydia-related bacterium that has been associated with adverse pregnancy outcomes in human. However, there is a lack of knowledge regarding how W. chondrophila infection spreads, its ability to elicit an immune response and induce pathology. A murine model of genital infection was developed to investigate the pathogenicity and immune response associated with a W. chondrophila infection. Genital inoculation of the bacterial agent resulted in a dose-dependent infection that spread to lumbar lymph nodes and successively to spleen and liver. Bacterial-induced pathology peaked on day 14, characterized by leukocyte infiltration (uterine horn, liver, and spleen), necrosis (liver) and extramedullary hematopoiesis (spleen). Immunohistochemistry demonstrated the presence of a large number of W. chondrophila in the spleen on day 14. Robust IgG titers were detected by day 14 and remained high until day 52. IgG isotypes consisted of high IgG2a, moderate IgG3 and no detectable IgG1, indicating a Th1-associated immune response. This study provides the first evidence that W. chondrophila genital infection is capable of inducing a systemic infection that spreads to major organs, induces uterus, spleen, and liver pathology and elicits a Th1-skewed humoral response. This new animal model will help our understanding of the mechanisms related to intracellular bacteria-induced miscarriages, the most frequent complication of pregnancy that affects one in four women. |
| Databáze: | OpenAIRE |
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