Disparity of Cytochrome Utilization in Anodic and Cathodic Extracellular Electron Transfer Pathways of Geobacter sulfurreducens Biofilms
| Autor: | Shafeer Kalathil, Khoa H. Ly, Erwin Reisner, Nina Heidary, Xin Fang, Nikolay Kornienko, Heather F. Greer |
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| Přispěvatelé: | Kornienko, Nikolay [0000-0001-7193-2428], Kalathil, Shafeer [0000-0002-2001-2100], Fang, Xin [0000-0001-9275-0690], Reisner, Erwin [0000-0002-7781-1616], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cytochrome
Iron F100 Succinic Acid Cytochrome c Group Electrons Acetates 010402 general chemistry 01 natural sciences Biochemistry Catalysis Article Cathodic protection Electron transfer Colloid and Surface Chemistry Bacterial Proteins Extracellular Geobacter sulfurreducens Electrodes biology Chemistry Biofilm General Chemistry Electrochemical Techniques biology.organism_classification C700 0104 chemical sciences Anode Biofilms cardiovascular system Biophysics biology.protein lipids (amino acids peptides and proteins) Geobacter Oxidation-Reduction |
| Zdroj: | Journal of the American Chemical Society |
| ISSN: | 0002-7863 |
| Popis: | Extracellular electron transfer (EET) in microorganisms is prevalent in nature and has been utilized in functional bioelectrochemical systems. EET of Geobacter sulfurreducens has been extensively studied and has been revealed to be facilitated through c-type cytochromes, which mediate charge between the electrode and G. sulfurreducens in anodic mode. However, the EET pathway of cathodic conversion of fumarate to succinate is still under debate. Here, we apply a variety of analytical methods, including electrochemistry, UV-vis absorption and resonance Raman spectroscopy, quartz crystal microbalance with dissipation, and electron microscopy, to understand the involvement of cytochromes and other possible electron-mediating species in the switching between anodic and cathodic reaction modes. By switching the applied bias for a G. sulfurreducens biofilm coupled to investigating the quantity and function of cytochromes, as well as the emergence of Fe-containing particles on the cell membrane, we provide evidence of a diminished role of cytochromes in cathodic EET. This work sheds light on the mechanisms of G. sulfurreducens biofilm growth and suggests the possible existence of a nonheme, iron-involving EET process in cathodic mode. |
| Databáze: | OpenAIRE |
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