New insights into the Plasmodium vivax transcriptome using RNA-Seq
| Autor: | Nicholas J. White, Bruce Russell, Nicholas P. J. Day, Zbynek Bozdech, Lei Zhu, Sachel Mok, Anchalee Jaidee, Peter R. Preiser, Mallika Imwong, François Nosten |
|---|---|
| Přispěvatelé: | School of Biological Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Untranslated region 030231 tropical medicine Plasmodium vivax RNA-Seq Article Chromosomes Transcriptome 03 medical and health sciences 0302 clinical medicine parasitic diseases Transcriptional regulation Humans Plasmodium Vivax Gene Genetics Multidisciplinary biology Alternative splicing High-Throughput Nucleotide Sequencing RNA biology.organism_classification Science::Biological sciences [DRNTU] 030104 developmental biology RNA Protozoan |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep20498 |
| Popis: | Historically seen as a benign disease, it is now becoming clear that Plasmodium vivax can cause significant morbidity. Effective control strategies targeting P. vivax malaria is hindered by our limited understanding of vivax biology. Here we established the P. vivax transcriptome of the Intraerythrocytic Developmental Cycle (IDC) of two clinical isolates in high resolution by Illumina HiSeq platform. The detailed map of transcriptome generates new insights into regulatory mechanisms of individual genes and reveals their intimate relationship with specific biological functions. A transcriptional hotspot of vir genes observed on chromosome 2 suggests a potential active site modulating immune evasion of the Plasmodium parasite across patients. Compared to other eukaryotes, P. vivax genes tend to have unusually long 5′ untranslated regions and also present multiple transcription start sites. In contrast, alternative splicing is rare in P. vivax but its association with the late schizont stage suggests some of its significance for gene function. The newly identified transcripts, including up to 179 vir like genes and 3018 noncoding RNAs suggest an important role of these gene/transcript classes in strain specific transcriptional regulation. |
| Databáze: | OpenAIRE |
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