Fluorometric determination of serum and urinary aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography
| Autor: | Hajime Yoshimura, Hitoshi Hoshino, Tetsuo Shimmura, Emiko Kaneko, Hideki Obi, Shin-ichi Hatakeyama, Takao Yotsuyanagi, Makoto Sato |
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| Rok vydání: | 1998 |
| Předmět: |
inorganic chemicals
Metal ions in aqueous solution chemistry.chemical_element complex mixtures Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry Matrix (chemical analysis) Blood serum Column chromatography Aluminium Humans Micelles Chromatography Chemistry Spectrophotometry Atomic Organic Chemistry Reproducibility of Results Sodium Dodecyl Sulfate General Medicine Reversed-phase chromatography respiratory system Oxyquinoline respiratory tract diseases Kinetics Spectrometry Fluorescence Micellar liquid chromatography Metals Indicators and Reagents Aluminum Chromatography Liquid |
| Zdroj: | Journal of chromatography. A. 789(1-2) |
| ISSN: | 0021-9673 |
| Popis: | A new fluorometric method has been developed for the determination of aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography. The proposed method enabled the determination of aluminium down to 1 microgram/l in human serum and urine without preliminary deproteinization. The most remarkable point of this method is that only aluminium ion selectively responds among metal ions. The complex formation of aluminium in serum with 8-quinolinol was completed within a few minutes at room temperature. The serum matrix and aluminium chelate were separated on a reversed-phase column with an eluent containing acetonitrile, sodium dodecylsulfate, and Triton X-100, and aluminium chelate was detected at Ex 370 nm, Em 504 nm. The values obtained by this method were in good agreement with those of Zeeman graphite-furnace atomic absorption spectrometry. The proposed method will provide a simple and rapid technique for the determination aluminium in medical fields. |
| Databáze: | OpenAIRE |
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