Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis—Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
| Autor: | Mohamad Yasmin, Laura J Rojas, Steven H Marshall, Andrea M Hujer, Anna Cmolik, Emma Marshall, Helen W Boucher, Alejandro J Vila, Maxime Soldevila, Seydina M Diene, Jean-Marc Rolain, Robert A Bonomo |
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| Přispěvatelé: | Louis Stokes Cleveland Veterans Affairs Medical Center, CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA., Tufts Medical Center, Boston, Massachusetts, USA., Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, Argentina., Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Case Western Reserve University School of Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Infectious Diseases Oncology [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Major Article [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| Zdroj: | Open Forum Infect Dis Open Forum Infectious Diseases Open Forum Infectious Diseases, 2023, 10 (2), pp.ofad014. ⟨10.1093/ofid/ofad014⟩ |
| ISSN: | 2328-8957 |
| Popis: | BackgroundElizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen.MethodsWhole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, blaBlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B.ResultsWGS identified the organism as E. anophelis. MBL genes blaBlaB-1 and blaGOB-26 were identified, in addition to blaCME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM.ConclusionsUsing WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection. |
| Databáze: | OpenAIRE |
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