Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System
| Autor: | Charles Manceau, Marie-Agnès Jacques, Marion Fischer-Le Saux, Salwa Essakhi, Sophie Bonneau, Sophie Cesbron |
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| Přispěvatelé: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Direction Generale de l'Armement (REI project) [2010 34007] |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Xanthomonas Genotype Sequence analysis [SDV]Life Sciences [q-bio] Molecular Sequence Data Sequence Homology Juglans Minisatellite Repeats Multiple Loci VNTR Analysis 01 natural sciences Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Plant Microbiology Tandem repeat Phylogenetics Type III Secretion Systems Cluster Analysis Phylogeny 030304 developmental biology Plant Diseases 0303 health sciences Ecology biology Phylogenetic tree Xanthomonas arboricola biology.organism_classification Variable number tandem repeat 010606 plant biology & botany Food Science Biotechnology Multilocus Sequence Typing |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (16), pp.5395-5410. ⟨10.1128/AEM.00835-15⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.00835-15⟩ |
| Popis: | Xanthomonas arboricola is conventionally known as a taxon of plant-pathogenic bacteria that includes seven pathovars. This study showed that X. arboricola also encompasses nonpathogenic bacteria that cause no apparent disease symptoms on their hosts. The aim of this study was to assess the X. arboricola population structure associated with walnut, including nonpathogenic strains, in order to gain a better understanding of the role of nonpathogenic xanthomonads in walnut microbiota. A multilocus sequence analysis (MLSA) was performed on a collection of 100 X. arboricola strains, including 27 nonpathogenic strains isolated from walnut. Nonpathogenic strains grouped outside clusters defined by pathovars and formed separate genetic lineages. A multilocus variable-number tandem-repeat analysis (MLVA) conducted on a collection of X. arboricola strains isolated from walnut showed that nonpathogenic strains clustered separately from clonal complexes containing Xanthomonas arboricola pv. juglandis strains. Some nonpathogenic strains of X. arboricola did not contain the canonical type III secretion system (T3SS) and harbored only one to three type III effector (T3E) genes. In the nonpathogenic strains CFBP 7640 and CFBP 7653, neither T3SS genes nor any of the analyzed T3E genes were detected. This finding raises a question about the origin of nonpathogenic strains and the evolution of plant pathogenicity in X. arboricola . T3E genes that were not detected in any nonpathogenic isolates studied represent excellent candidates to be those responsible for pathogenicity in X. arboricola . |
| Databáze: | OpenAIRE |
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