3D printing of chemical-empowered tendon stem/progenitor cells for functional tissue repair
| Autor: | Renjie Liang, Jinchun Ye, Chenqi Tang, Zi Yin, Xiaoyi Chen, Yanyan Zhao, Tao Zhang, Yuanhao Xie, Youguo Liao, Yanjie Zhang, Tingyun Lei, Weiliang Shen, Bo Zhou, Yangwu Chen, Yi Hong, Xiao Chen, Hong Zhang, Boon Chin Heng, Wei Ju, Chunmei Fan |
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| Rok vydání: | 2021 |
| Předmět: |
Biophysics
Bioengineering 02 engineering and technology Biology Rats Sprague-Dawley Tendons Biomaterials 03 medical and health sciences Tissue engineering In vivo medicine Animals Humans Progenitor cell 030304 developmental biology 0303 health sciences Reporter gene Tissue Engineering Stem Cells Regeneration (biology) Cell Differentiation 021001 nanoscience & nanotechnology In vitro Rats Tendon Cell biology medicine.anatomical_structure Mechanics of Materials Printing Three-Dimensional Ceramics and Composites 0210 nano-technology Ex vivo |
| Zdroj: | Biomaterials. 271:120722 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2021.120722 |
| Popis: | Tendon injuries are the leading cause of chronic debilitation to patients. Tendon stem/progenitor cells (TSPCs) are potential seed cells for tendon tissue engineering and regeneration, but TSPCs are prone to lose their distinct phenotype in vitro and specific differentiation into the tenocyte lineage is challenging. Utilizing small molecules in an ex vivo culture system may be a promising solution and can significantly improve the therapeutic applications of these cells. Here, by using an image-based, high-throughput screening platform on small molecule libraries, this study established an effective stepwise culture strategy for TSPCs application. The study formulated a cocktail of small molecules which effected proliferation, tenogenesis initiation and maturation phases, and significantly upregulated expression of various tendon-related genes and proteins in TSPCs, which were demonstrated by high-throughput PCR, ScxGFP reporter assay and immunocytochemistry. Furthermore, by combining small molecule-based culture system with 3D printing technology, we embedded living, chemical-empowered TSPCs within a biocompatible hydrogel to engineer tendon grafts, and verified their enhanced ability in promoting functional tendon repair and regeneration both in vivo and in situ. The stepwise culture system for TSPCs and construction of engineered tendon grafts can not only serve as a platform for further studies of underlying molecular mechanisms of tenogenic differentiation, but also provide a new strategy for tissue engineering and development of novel therapeutics for clinical applications. |
| Databáze: | OpenAIRE |
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