Eco1 is important for DNA damage repair inS. cerevisiae
| Autor: | Matthew Goering, Sue L. Jaspersen, Scarlett Gard, Adrian J. McNairn, Shuai Lu, Jennifer L. Gerton, Bo Xiong |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA Repair Cohesin complex Chromosomal Proteins Non-Histone DNA repair Mutant Mitosis Cell Cycle Proteins Saccharomyces cerevisiae Protein Serine-Threonine Kinases Biology medicine.disease_cause Chromosome segregation Bleomycin Cell Cycle News & Views Acetyltransferases Chromosome Segregation Report medicine DNA Breaks Double-Stranded Phosphorylation Roberts syndrome Molecular Biology Genetics Mutation Antibiotics Antineoplastic Cohesin X-Rays Nuclear Proteins Cell Biology medicine.disease Checkpoint Kinase 2 Developmental Biology |
| Zdroj: | Cell Cycle. 9:3335-3347 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/cc.9.16.12673 |
| Popis: | The cohesin network has an essential role in chromosome segregation, but also plays a role in DNA damage repair. Eco1 is an acetyltransferase that targets subunits of the cohesin complex and is involved in both the chromosome segregation and DNA damage repair roles of the network. Using budding yeast as a model system, we find that mutations in Eco1, including a genocopy of a human Roberts syndrome allele, do not cause gross defects in chromosome cohesion. We examined how mitotic and meiotic DNA damage repair is affected by mutations in Eco1. Strains containing mutations in Eco1 are sensitive to DNA damaging agents that cause double-strand breaks, such as X-rays and bleomycin. While meiotic crossing over is relatively unaffected in strains containing the Roberts mutation, reciprocal mitotic crossovers occur with extremely low frequency in this mutant background. Our results suggest that Eco1 promotes the reciprocal exchange of chromosome arms and maintenance of heterozygosity during mitosis. |
| Databáze: | OpenAIRE |
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