Interrogating biomineralization one amino acid at a time: amplification of mutational effects in protein-aided titania morphogenesis through reaction-diffusion control
| Autor: | Karthik Pushpavanam, François Baneyx, Brittney Hellner |
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| Rok vydání: | 2021 |
| Předmět: |
Biomineralization
Morphology (linguistics) Surface Properties Green Fluorescent Proteins Nanoparticle Catalysis Metal Diffusion chemistry.chemical_compound Materials Chemistry Amino Acids Particle Size chemistry.chemical_classification Titanium Metals and Alloys General Chemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Amino acid chemistry visual_art Yield (chemistry) Mutation Ceramics and Composites visual_art.visual_art_medium Biophysics Particle Agarose Peptides |
| Zdroj: | Chemical communications (Cambridge, England). 57(39) |
| ISSN: | 1364-548X |
| Popis: | To emulate the control that biomineralizing organisms exert over reactant transport, we construct a countercurrent reaction-diffusion chamber in which an agarose hydrogel regulates the fluxes of inorganic precursor and precipitating solid-binding protein. We show that the morphology of the bioprecipitated titania can be changed from monolithic to interconnected particle networks and dispersed nanoparticles either by decreasing reaction time or by increasing agarose weight percentage at constant precursor and protein concentrations. More strikingly, protein variants with one or two substitutions in their metal oxide-binding domain yield unique peripheral morphologies (needles, threads, plates, and peapods) with distinct crystallography and photocatalytic activity. Our results suggest that diffusional control can magnify otherwise subtle mutational effects in biomineralizing proteins and provide a path for the green synthesis of morphologically and functionally diverse inorganic materials. |
| Databáze: | OpenAIRE |
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