Popis: |
Multiple antibacterial agents have been mixed and used as an intracanal medicament to eliminate E.faecalis, which has been most frequently identified in the cases of failed root canal treatment and periapical lesions. This study is aimed at using newer intracanal medicament Nitrofurantoin and Ozonated oil plus calcium hydroxide against E.faecalis bacteria. Aim: To compare the antimicrobial efficacy of newer intracanal medicament Nitrofurantoin and Ozonated oil plus calcium hydroxide on E.faecalis - an in vitro study. Materials and methodology: Thirty extracted premolars were collected and instrumented with rotary files (size 25-4% taper). Then, root canals were contaminated with a bacterial solution of E.faecalis for 21 days, and divided into two groups. In the root canals of group A nitrofurantoin intracanal medicament was placed and in group B ozonated oil plus calcium hydroxide intracanal medicament was placed and sealed for 7 days. Then on the 8th day, the contaminated root canals were flushed with normal saline to remove the medicament. To evaluate the degree of contamination, the specimens of the dentin chips from the full length of the root canal were harvested using a sterile rotary instrument (size 25- 6% taper) and placed in BHI broth and same were subjected for dilution and colony count after 24 hours. Data was recorded and subjected to statistical analysis. Results and conclusion: within the limits of the study, Nitrofurantoin showed greater antibacterial efficacy on E.faecalis when compared to the mixture of Ozonated oil plus calcium hydroxide as an intracanal medicament. |