Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes
| Autor: | Halina Cernohorska, Jiri Rubes, Svatava Kubickova, Giuseppe Enne, Dino Di Berardino, Miluse Vozdova, Gianfranco Coppola, Giuseppe Coppola |
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| Přispěvatelé: | DI BERARDINO, Dino, Vozdova, M, Kubickova, S, Cernohorska, H, Coppola, Gianfranco, Coppola, Gf, Enne, G, Rubes, J. |
| Rok vydání: | 2003 |
| Předmět: |
Male
Sex Determination Analysis X Chromosome Buffaloes Sexing Biology Andrology Animal science Species Specificity FISH spermatozoa Y Chromosome Genetics medicine Capra hircus Animals sexing Ovis In Situ Hybridization In Situ Hybridization Fluorescence Fluorescent Dyes Sheep medicine.diagnostic_test urogenital system Goats Lasers Embryo Cell Biology biology.organism_classification Sperm Cattle Bubalus Ploidy Developmental Biology Fluorescence in situ hybridization |
| Zdroj: | Molecular Reproduction and Development. 67:108-115 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.20016 |
| Popis: | River buffalo, sheep, and goat spermatozoa were cross-hybridized using double color fluorescence in situ hybridization (FISH) with bovine Xcen- and Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae. Before sperm analysis, the probes were hybridized on metaphase chromosomes of each species, as control. Frozen-thawed spermatozoa of cattle, river buffalo, sheep, and goat were decondensed in suspension with 5 mM DTT. Sperm samples obtained from three individuals of each species were investigated, more than 1,000 spermatozoa were scored in each animal. FISH analysis of more than 12,000 sperm revealed high level of sperm with X- or Y-signals in all of the species investigated, indicating FISH efficiency over 99%. Significant interspecific differences were detected in the frequency of aberrant spermatozoa (aneuploid and diploid) between goat (0.393%) and sheep (0.033%) (P |
| Databáze: | OpenAIRE |
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