Potential Diagnostic Value of Red Blood Cells α-Synuclein Heteroaggregates in Alzheimer’s Disease
| Autor: | Roberto Ceravolo, Ubaldo Bonuccelli, Maria Letizia Trincavelli, Linda Giampietri, Lucia Petrozzi, Paolo Libertini, Annalisa Lo Gerfo, Daniela Frosini, Filippo Sean Giorgi, Filippo Baldacci, Gloria Tognoni, Valentina Nicoletti, Claudia Martini, Deborah Pietrobono, Laura Betti, Simona Daniele, Rebecca Piccarducci, Elena Donadio, Gabriele Siciliano |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Neurology Erythrocytes animal diseases α-Synuclein heterocomplexes Neuroscience (miscellaneous) Red blood cells Cohort Studies 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cerebrospinal fluid Alzheimer Disease Internal medicine medicine Dementia Humans Pathological Aged α-Synuclein business.industry Alzheimer’s disease Tau β-Amyloid Neurodegeneration Middle Aged medicine.disease Pathophysiology 030104 developmental biology Endocrinology nervous system ROC Curve alpha-Synuclein Biomarker (medicine) Female Alzheimer's disease business 030217 neurology & neurosurgery Biomarkers |
| Popis: | A plethora of complex misfolded protein combinations have been found in Alzheimer disease (AD) brains besides the classical pathological hallmarks. Recently, α-synuclein (α-syn) and its heterocomplexes with amyloid-β (Aβ) and tau have been suggested to be involved in the pathophysiological processes of neurodegenerative diseases. These pathological features are not limited to the brain, but can be also found in peripheral fluids. In this respect, red blood cells (RBCs) have been suggested as a good model to investigate the biochemical alterations of neurodegeneration. Our aim is to find whether RBC concentrations of α-syn and its heterocomplexes (i.e., α-syn/Aβ and α-syn/tau) were different in AD patients compared with healthy controls (HC). The levels of homo- and heteroaggregates of α-syn, Aβ and tau, were analyzed in a cohort of AD patients at early stage either with dementia or prodromal symptoms (N = 39) and age-matched healthy controls (N = 39). All AD patients received a biomarker-based diagnosis (low cerebrospinal fluid levels of Aβ peptide combined with high cerebrospinal fluid concentrations of total tau and/or phospho-tau proteins; alternatively, a positivity to cerebral amyloid-PET scan). Our results showed lower concentrations of α-syn and its heterocomplexes (i.e., α-syn/Aβ and α-syn/tau) in RBCs of AD patients with respect to HC. RBC α-syn/Aβ as well as RBC α-syn/tau heterodimers discriminated AD participants from HC with fair accuracy, whereas RBC α-syn concentrations differentiated poorly the two groups. Although additional investigations are required, these data suggest α-syn heteroaggregates in RBCs as potential tool in the diagnostic work-up of early AD diagnosis. |
| Databáze: | OpenAIRE |
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