Cytotoxic and Apoptotic Effects of Three Types of Silver-Iron Oxide Binary Hybrid Nanoparticles
| Autor: | Sara Rasoul-Amini, Ali Moghadam, Ali Niazi, Alireza Ebrahiminezhad, Narjes Ebrahimi, Nasrollah Erfani, Younes Ghasemi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Silver Cell Survival Metal Nanoparticles Pharmaceutical Science Nanoparticle Antineoplastic Agents Apoptosis 02 engineering and technology Ferric Compounds Silver nanoparticle Magnetics 03 medical and health sciences chemistry.chemical_compound Bcl-2-associated X protein Humans Cytotoxic T cell Cytotoxicity bcl-2-Associated X Protein biology Hep G2 Cells 021001 nanoscience & nanotechnology Molecular biology Up-Regulation 030104 developmental biology chemistry Cancer cell biology.protein Biophysics Magnetic nanoparticles 0210 nano-technology Iron oxide nanoparticles Transcription Factors Biotechnology |
| Zdroj: | Current Pharmaceutical Biotechnology. 17:1049-1057 |
| ISSN: | 1389-2010 |
| DOI: | 10.2174/1389201017666160907143807 |
| Popis: | Background: Multifunctional nanostructures have received great deal of attention in biomedical area due to their capabilities in the development of new therapeutic and diagnostic agents. Silver and iron oxide nanoparticles, owing to their specific characteristics, are considered to develop bifunctional hybrid nanoparticles for magnetic delivery of silver nanoparticles as cytotoxic agent toward cancer cells. Objective: This study was designed to explore the in-vitro cytotoxic and apoptotic activities of three different silver-iron oxide binary hybrid nanoparticles on human liver hepatocellular carcinoma cell line. Method: Three different silver-iron oxide binary hybrid nanoparticles were synthesized and characterized through the designed procedures. Apoptosis induction was investigated through flow cytometry and the influence on bax gene expression level was analyzed using quantitative real time polymerase chain reaction. Results: All the three types of silver-iron oxide hybrid nanoparticles (possessing different characteristics) exhibited cytotoxic and apoptotic effects. Furthermore, the up regulation of bax gene expression suggested the involvement of the intrinsic pathway of apoptosis. Some of the transcription regulators which could interact with bax gene promoter were analyzed and found out to be mostly contributed in the stress responses. Among the test nanoparticles, the strongest cytotoxic and apoptotic effect was induced by the binary hybrid nanoparticle which was synthesized with glucose as reducing agent; suggesting that the biological activity was affected by different characteristics of the designed nanoparticles. Conclusion: Combined properties of silver and magnetic nanoparticles in the binary hybrid nanoparticles, provide a great potential to be exploited in the cancer therapy, where the combination of cytotoxicity and magnetic targeting is desired. |
| Databáze: | OpenAIRE |
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