Positive Influence of Hyaluronate on Cell Vitality of Human Conjunctival Cells after Alkali Injuries
| Autor: | Otto Schmut, Gabriele Trummer, Christoph Faschinger, Dieter Franz Rabensteiner, Helga Spitzenberger, Andreas Wedrich |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Conjunctiva Dose-Response Relationship Drug business.industry Cell Cell Count General Medicine Alkalies Vitality Statistics Nonparametric Sensory Systems Ophthalmology medicine.anatomical_structure Adjuvants Immunologic Immunology medicine Humans Drug Interactions Hyaluronic Acid business Cells Cultured |
| Zdroj: | Ophthalmologica. 224:217-223 |
| ISSN: | 1423-0267 0030-3755 |
| Popis: | Background:To analyse the influence of hyaluronate (HA) on the cell vitality of human conjunctival cells after chemical injuries from NaOH or alkali-containing household cleaners. Methods:Human conjunctival cells (Chang cells) were incubated either with NaOH solutions or dilutions of household cleaners. HA (0.025%) was added to the cell culture medium of part of the cells subsequent to chemical injuring. After 24 h the cell vitality of the HA-incubated cells, determined by MTS assay (Cell Titer 96® Aqueous One Solution Cell Proliferation Assay), was compared to the control cells without HA addition. Results: The addition of HA to the cell culture medium subsequent to chemical injuring resulted in a significantly lower decrease in cell vitality (mean difference: 11.79; p = 0.00; CI = 98%). Conclusion: Because HA attenuates the decrease in cell vitality of alkali-damaged human conjunctival cells, formulations containing HA may be recommended for the treatment of chemical injuries to the anterior part of the eye. |
| Databáze: | OpenAIRE |
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