Chitosan-Coated Nanoparticles: Effect of Chitosan Molecular Weight on Nasal Transmucosal Delivery
| Autor: | Fabio Sonvico, Adriana Raffin Pohlmann, Stefania Pigana, Tanira Aguirre, Franciele Aline Bruinsmann, Gabriele Dadalt Souto, Gaia Colombo, Magno da Silva Marques, Gabriela Garrastazu Pereira, Silvia Stanisçuaski Guterres, Laura Tiozzo Fasiolo, Annalisa Bianchera |
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| Rok vydání: | 2019 |
| Předmět: |
Chitosan
CNS disorders Mucoadhesion Nanocapsules Nasal permeability Nose-to-brain Simvastatin Drug delivery to the brain lcsh:RS1-441 Pharmaceutical Science Nanoparticle 02 engineering and technology 030226 pharmacology & pharmacy Article Nanocapsules NO lcsh:Pharmacy and materia medica Chitosan 03 medical and health sciences chemistry.chemical_compound CNS disorders 0302 clinical medicine Mucoadhesion LS7_3 simvastatin Surface charge nasal permeability nanocapsules Permeation 021001 nanoscience & nanotechnology chemistry nose-to-brain Particle size chitosan 0210 nano-technology mucoadhesion biomaterials Biomedical engineering |
| Zdroj: | Pharmaceutics Volume 11 Issue 2 Pharmaceutics, Vol 11, Iss 2, p 86 (2019) |
| Popis: | Drug delivery to the brain represents a challenge, especially in the therapy of central nervous system malignancies. Simvastatin (SVT), as with other statins, has shown potential anticancer properties that are difficult to exploit in the central nervous system (CNS). In the present work the physico–chemical, mucoadhesive, and permeability-enhancing properties of simvastatin-loaded poly-ε-caprolactone nanocapsules coated with chitosan for nose-to-brain administration were investigated. Lipid-core nanocapsules coated with chitosan (LNCchit) of different molecular weight (MW) were prepared by a novel one-pot technique, and characterized for particle size, surface charge, particle number density, morphology, drug encapsulation efficiency, interaction between surface nanocapsules with mucin, drug release, and permeability across two nasal mucosa models. Results show that all formulations presented adequate particle sizes (below 220 nm), positive surface charge, narrow droplet size distribution (PDI < 0.2), and high encapsulation efficiency. Nanocapsules presented controlled drug release and mucoadhesive properties that are dependent on the MW of the coating chitosan. The results of permeation across the RPMI 2650 human nasal cell line evidenced that LNCchit increased the permeation of SVT. In particular, the amount of SVT that permeated after 4 hr for nanocapsules coated with low-MW chitosan, high-MW chitosan, and control SVT was 13.9 ± 0.8 μg, 9.2 ± 1.2 µg, and 1.4 ± 0.2 µg, respectively. These results were confirmed by SVT ex vivo permeation across rabbit nasal mucosa. This study highlighted the suitability of LNCchit as a promising strategy for the administration of simvastatin for a nose-to-brain approach for the therapy of brain tumors. |
| Databáze: | OpenAIRE |
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