Radioimmunoassays of prostaglandin F-2-alpha and prostaglandin F-2-alpha-main urinary metabolite with prostaglandin-125-I-tyrosine methylester amide
| Autor: | Katsuhiro Imaki, Toshio Hanyu, Shiro Ohki, Nobuhiko Nakazawa, Fumio Hirata |
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| Rok vydání: | 1974 |
| Předmět: |
endocrine system
medicine.medical_specialty Urinary system Metabolite Radioimmunoassay Prostaglandin Cross Reactions Tritium Biochemistry Iodine Radioisotopes chemistry.chemical_compound Endocrinology Internal medicine medicine Methods Animals Humans Tyrosine Bovine serum albumin Antiserum biology Chemistry Immune Sera Serum Albumin Bovine respiratory system Charcoal cardiovascular system biology.protein Prostaglandins Scintillation Counting lipids (amino acids peptides and proteins) Rabbits Antibody hormones hormone substitutes and hormone antagonists |
| Zdroj: | Prostaglandins. 6(2) |
| ISSN: | 0090-6980 |
| Popis: | Radioimmunoassays for measuring prostaglandin F 2α (PGF 2α ) and 5α, 7α-dihydroxy-11-keto tetranorprosta-1,16-dioic acid, PGF 2α -main urinary metabolite (PGF 2α -MUM), with 125 I-tyrosine methylester amide (TMA) of PGF 2α and PGF 2α -MUM were developed. Antibody to PGF 2α was produced in rabbits immunized with conjugates of PGF 2α coupled to bovine serum albumine. Antibody to PGF 2α -MUM was also produced in rabbits immunized with conjugates of PGF 2α -MUM coupled to bovine serum albumin. PGF 2α - 125 I-TMA had an affinity to antiserum to PGF 2α . PGF 2α -MUM- 125 I-TMA also responded to antiserum to PGF 2α -MUM. |
| Databáze: | OpenAIRE |
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