Identification and characterization of a cytotoxic T cell epitope of hepatitis C virus presented by HLA-B*3501 in acute hepatitis
| Autor: | Kunitada Shimotohno, Katsuaki Tanaka, Yasushi Shiratori, Takashi Sakaguchi, Masaaki Ibe, Yoshitomo Chujoh, Kiyoshi Miwa, Torahiko Tanaka, Masafumi Takiguchi, Satoru Saito, Shumpei Yokota, Masao Omata |
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| Rok vydání: | 1998 |
| Předmět: |
Hepatitis C virus
T cell Epitopes T-Lymphocyte Hepacivirus Biology medicine.disease_cause Epitope Virus Cell Line Virology medicine Humans Cytotoxic T cell Antigen Presentation Hepatitis C Hepatitis C Chronic medicine.disease Molecular biology CTL Epitope mapping medicine.anatomical_structure Acute Disease HLA-B35 Antigen Hepatitis C Antigens Peptides Epitope Mapping T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of General Virology. 79:1735-1744 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | In order to clarify the role of cytotoxic T lymphocytes (CTL) in hepatitis C virus (HCV) infection, an HLA-B35-restricted cytotoxic T cell epitope of HCV was identified using a strategy called reverse immunogenetics. Twenty-eight of 53 HCV peptides carrying two anchor residues were selected as HLA-B*3501 binding peptides. These peptides were used to induce the specific cytotoxic T cells in peripheral blood lymphocytes from a patient with acute hepatitis C. Six HLA-B*3501 binding peptides induced the peptide-specific CTL. One (HPNIEEVAL) of five peptides was confirmed as the epitope by the specific T cell clones. A sequence identical to the epitope was detected in isolates of the virus from the patient and a strong CTL response to this epitope was observed in the acute phase of hepatitis C but not in the recovery phase. The specific CTL for this epitope were not detected in peripheral blood lymphocytes from patients with chronic hepatitis C. Together these results suggest that the CTL specific for this epitope have an important role in the elimination of the virus in the patient. |
| Databáze: | OpenAIRE |
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