Pinosylvin and monomethylpinosylvin, constituents of an extract from the knot of Pinus sylvestris, reduce inflammatory gene expression and inflammatory responses in vivo
| Autor: | Christer Eckerman, Mirka Laavola, Bjarne Holmbom, Eeva Moilanen, Tiina Leppänen, Riina Nieminen |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.drug_class
Pinosylvin Anti-Inflammatory Agents Gene Expression Nitric Oxide Synthase Type II Inflammation Carrageenan Nitric Oxide Anti-inflammatory Nitric oxide Proinflammatory cytokine Cell Line chemistry.chemical_compound Mice In vivo Stilbenes medicine Animals Humans Chemokine CCL2 EC50 biology Interleukin-6 Plant Extracts Macrophages NF-kappa B Pinus sylvestris General Chemistry Wood Nitric oxide synthase HEK293 Cells chemistry Biochemistry biology.protein medicine.symptom General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry. 63(13) |
| ISSN: | 1520-5118 |
| Popis: | Scots pine (Pinus sylvestris) is known to be rich in phenolic compounds, which may have anti-inflammatory properties. The present study investigated the anti-inflammatory effects of a knot extract from P. sylvestris and two stilbenes, pinosylvin and monomethylpinosylvin, isolated from the extract. Inflammation is characterized by increased release of pro-inflammatory and regulatory mediators including nitric oxide (NO) produced by the inducible nitric oxide synthase (iNOS) pathway. The knot extract (EC50 values of 3 and 3 μg/mL) as well as two of its constituents, pinosylvin (EC50 values of 13 and 15 μM) and monomethylpinosylvin (EC50 values of 8 and 12 μM), reduced NO production and iNOS expression in activated macrophages. They also inhibited the production of inflammatory cytokines IL-6 and MCP-1. More importantly, pinosylvin and monomethylpinosylvin exerted a clear anti-inflammatory effect (80% inhibition at the dose of 100 mg/kg) in the standard in vivo model, carrageenan-induced paw inflammation in the mouse, with the effect being comparable to that of a known iNOS inhibitor L-NIL. The results reveal that the Scots pine stilbenes pinosylvin and monomethylpinosylvin are potential anti-inflammatory compounds. |
| Databáze: | OpenAIRE |
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