Quantitative proteomic profiling of targeted proteins associated with Loki Zupa Decoction Treatment in OVA-Induced asthmatic mice
| Autor: | Xueyi Zhu, Jie Cui, Tulake Wuniqiemu, Jingcheng Dong, Muhammadjan Abduwaki, Mohammadtursun Nabijan, Jingjing Qin, Mammat Nurahmat, Weifeng Tang, Fangzhou Teng, La Yi, Ying Wei |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Ovalbumin Quantitative proteomics Anti-Inflammatory Agents Receptors Cell Surface Decoction Pharmacology Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Drug Discovery Animals Medicine Anti-Asthmatic Agents Medicine Chinese Traditional Pathological 030304 developmental biology Inflammation Mice Inbred BALB C 0303 health sciences Lung business.industry Proteomic Profiling Significant difference respiratory system Asthma Disease Models Animal medicine.anatomical_structure 030220 oncology & carcinogenesis Cytokines Female business Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 266:113343 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2020.113343 |
| Popis: | Loki Zupa (LKZP) decoction is one of the herbal prescriptions in traditional Uyghur medicine, which is commonly used for treating airway abnormality. However, underlying pathological mechanism and pathways involved has not been well studied.In this paper, we aim to further confirmed the anti-inflammatory and anti-fibrotic role of LKZP decoction in airway, and uncover the passible mechanism involved via comprehensive quantitative proteomic DIA-MS analysis.Mice asthmatic model was established with sensitizing and challenging with OVA. Lung function, pathological status, and inflammatory cytokines were assessed. Total of nine lung tissues were analyzed using proteomic DIA-MS analysis and 18 lung tissues were subjected to PRM validation.Total of 704 differentially expressed proteins (DEPs) (363 up regulated, 341 down regulated) were quantified in comparison of asthmatic and healthy mice, while 152 DEPs (91 up regulated, 61 down regulated) were quantified in LKZP decoction treated compared to asthmatic mice. Total of 21 proteins were overlapped between three groups. ECM-receptor interaction was significantly enriched and commonly shared between downregulated DEPs in asthma and upregulated DEPs in LKZP decoction treated mice. Total of 20 proteins were subjected to parallel reaction monitoring (PRM) analysis and 16 of which were quantified. At last, two proteins, RMB 10 and COL6A6, were validated with significant difference (P 0.001) in protein abundance.Our results suggest that attenuated airway inflammation and fibrosis caused by LKZP decoction may associated with ECM-receptor interaction and RMB 10 and COL6A6 may be targeted by LKZP decoction in OVA-induced asthmatic mice. |
| Databáze: | OpenAIRE |
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